The pigment-protein composition of two mesophyl chloroplast fractions from maize inbred lines II 346 and F 7 and its alterations under phosphorylation have been studied. In virtue of differences in the composition of photosystem II (PS II) and PS II light-harvesting chlorophyll a/b-protein complex (LHC II) it has been shown that fraction 70,000 g origins from grana marginal regions and fraction 100,000 g is from stroma lamellae. Migration of LHC II occurs both in marginal and stromal fractions but PS II migration, only in marginal fractions. There are some differences in peculiarities of these processes between the two maize inbred lines which can be determined genetically.
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J Agric Food Chem
October 2024
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
This study investigates the properties and potential applications of phycoerythrin 545, a naturally occurring light-harvesting pigment protein from . Phycoerythrin 545, characterized by its bright red color and maximum absorption wavelength at 545 nm, was extracted using freeze-thawing methods, further purified, and analyzed using chromatographic, spectroscopic techniques, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Phycoerythrin 545 consists of two subunits, primarily α and β, but lacks the γ subunit, and is stable at 4 °C within a pH range of 3-10.
View Article and Find Full Text PDFInt J Mol Sci
March 2024
Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
Photosystem I (PSI) is one of the two main pigment-protein complexes where the primary steps of oxygenic photosynthesis take place. This review describes low-temperature frequency-domain experiments (absorption, emission, circular dichroism, resonant and non-resonant hole-burned spectra) and modeling efforts reported for PSI in recent years. In particular, we focus on the spectral hole-burning studies, which are not as common in photosynthesis research as the time-domain spectroscopies.
View Article and Find Full Text PDFPlants (Basel)
April 2024
Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
Salt stress significantly impacts the functions of the photosynthetic apparatus, with varying degrees of damage to its components. Photosystem II (PSII) is more sensitive to environmental stresses, including salinity, than photosystem I (PSI). This study investigated the effects of different salinity levels (0 to 200 mM NaCl) on the PSII complex in isolated thylakoid membranes from hydroponically grown pea ( L.
View Article and Find Full Text PDFBiotechnol Biofuels Bioprod
March 2024
Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, 04763, Korea.
Background: Photosynthetic microalgae are known for their sustainable and eco-friendly potential to convert carbon dioxide into valuable products. Nevertheless, the challenge of self-shading due to high cell density has been identified as a drawback, hampering productivity in sustainable photoautotrophic mass cultivation. To address this issue, mutants with altered pigment composition have been proposed to allow a more efficient light diffusion but further study on the role of the different pigments is still needed to correctly engineer this process.
View Article and Find Full Text PDFMembranes (Basel)
November 2023
Faculty of Physics, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
A transmembrane difference in the electrochemical potentials of protons (Δμ) serves as a free energy intermediate in energy-transducing organelles of the living cell. The contributions of two components of the Δμ (electrical, Δψ, and concentrational, ΔpH) to the overall Δμ value depend on the nature and lipid composition of the energy-coupling membrane. In this review, we briefly consider several of the most common instrumental (electrometric and EPR) methods for numerical estimations of Δψ and ΔpH.
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