By electron microscopy using immunocytochemical reactions on actin and EGF-R with mild glycerinization it became possible to reveal a thick layer of actin filaments under the apical membrane within the first minutes of the impact of EGF on A431 cells. Keratin fibrils being located near the nucleus are transported apart from the membrane. It is supposed that this process is connected with retardation of capping on principles of competition.
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