The aim of the present study was to evaluate the effect of culture conditions on extracellular protease biosynthesis by Streptomyces spp. G157. It was found that there was optimum expression of extracellular proteases in a medium of soyabean meal (2%), mannitol (2%) at a pH of 7.2 and temperature of 28 degrees C. Among the sugars glucose and starch had a greater repressive effect than the others. Phosphate was found to have an inhibitory effect on protease biosynthesis.
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