A new approach for modification of target DNAs with tandems of derivatives of short oligonucleotides was suggested that allows highly selective modification of perfect duplexes only. At physiological temperatures, the efficiency of DNA modification by a dodecanucleotide alkylating agent was demonstrated to be the same for both perfect and mismatch-containing duplexes, whereas the tetranucleotide reagent in the presence of two flanking effectors alkylated with high selectivity the target DNA in the perfect duplex only.

Download full-text PDF

Source

Publication Analysis

Top Keywords

short oligonucleotides
8
[nucleic acids
4
acids interactions
4
interactions short
4
short oligonucleotide
4
oligonucleotide derivatives
4
derivatives tandem
4
tandem short
4
oligonucleotides highly
4
highly sensitive
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!