AI Article Synopsis
- A study analyzed 152 Thermus isolates from hot springs around the world for the presence of the TaqI restriction enzyme.
- 27 of these isolates showed evidence of TaqI isoschizomers, specifically from hot springs in countries like New Zealand, Iceland, and the USA.
- Six isolates, identified as specific Thermus species, underwent further analysis revealing differences in their properties like magnesium needs and thermal stability.
Article Abstract
One-hundred-and-fifty-two isolates of the genus Thermus, collected from hot springs on four continents, were screened for evidence of the presence of the thermophilic Type II restriction endonuclease TaqI (T/CGA). The presence of isoschizomers of TaqI in 27 of the isolates, originating from hot springs in New Zealand, Iceland, USA, Japan, mainland Portugal and the island of São Miguel in the Azores, is reported. Six of the TaqI-containing isolates from diverse geographical locations, identified by means of DNA/DNA homology and 16S rRNA sequence alignment as belonging to the Thermus species T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus and T. brockianus, were selected for comparative studies. The TaqI isoschizomers from each of the six isolates were partially purified. They differed in their magnesium ion requirements, isoelectric points, subunit molecular masses and thermal stability.
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| http://dx.doi.org/10.1099/00221287-144-1-167 | DOI Listing |
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