The indirect peroxidase method was employed to study the endocrine pancreas of the Cape fur seal. Immunoreactivity to insulin was confined to the cores of the islets and the insulin cells were more abundant than the other endocrine cell types, which occurred mainly in the mantles of the islets. Of these, glucagon cells were the most numerous, followed by somatostatin and pancreatic polypeptide (PP) cells. The latter were observed in the mantles of the islets and scattered in the exocrine tissue of the duodenal lobe. The marked variation in the shape and the distribution of the endocrine cells in the mantles of the islets seen in the pancreas of the seal, seems to be typical of carnivorous species like the cat and dog.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
PDX1+ cell budding morphogenesis in a stem cell-derived islet spheroid system.
Nat Commun
July 2024
Life Sciences Institute, Departments of Cellular & Physiological Sciences and Surgery, University of British Columbia, Vancouver, BC, Canada.
Remarkable advances in protocol development have been achieved to manufacture insulin-secreting islets from human pluripotent stem cells (hPSCs). Distinct from current approaches, we devised a tunable strategy to generate islet spheroids enriched for major islet cell types by incorporating PDX1+ cell budding morphogenesis into staged differentiation. In this process that appears to mimic normal islet morphogenesis, the differentiating islet spheroids organize with endocrine cells that are intermingled or arranged in a core-mantle architecture, accompanied with functional heterogeneity.
View Article and Find Full Text PDFConflicting Views About Interactions Between Pancreatic α-Cells and β-Cells.
Diabetes
December 2023
Joslin Diabetes Center, Harvard Medical School, Boston, MA.
In type 1 diabetes, the reduced glucagon response to insulin-induced hypoglycemia has been used to argue that β-cell secretion of insulin is required for the full glucagon counterregulatory response. For years, the concept has been that insulin from the β-cell core flows downstream to suppress glucagon secretion from the α-cells in the islet mantle. This core-mantle relationship has been supported by perfused pancreas studies that show marked increases in glucagon secretion when insulin was neutralized with antisera.
View Article and Find Full Text PDFPathogenesis of an experimental mycobacteriosis in an apple snail.
Front Immunol
October 2023
IHEM, CONICET, Universidad Nacional de Cuyo, Mendoza, Argentina.
In this work, we aimed at investigating cell and tissue responses of the apple snail , following the inoculation of the zoonotic pathogen . Different doses were tested (10, 20, 65, and 100 M CFU) and the mortality rate was negligible. The histopathogenesis was followed at 4, 9, and 28 days after inoculation.
View Article and Find Full Text PDFThe glucagon receptor antagonist desHisProGlu-glucagon(LysPAL) alters alpha-cell turnover and lineage in mice, but does not cause alpha-cell hyperplasia.
Mol Cell Endocrinol
June 2023
Centre for Diabetes, Ulster University, Coleraine, Northern Ireland, UK. Electronic address:
Objective: Glucagon receptor (GCGR) antagonism elicits antihyperglycemic effects in rodents and humans. The present study investigates whether the well characterised peptide-based GCGR antagonist, desHisProGlu-glucagon (LysPAL), alters alpha-cell turnover or identity in mice.
Methods: Multiple low-dose streptozotocin (STZ) treated (50 mg/kg bw, 5 days) transgenic Glu;ROSA26-eYFP mice were employed.
Fabrication of functional rat pseudo-islets after cryopreservation of pancreatic islets or dispersed islet cells.
J Tissue Eng Regen Med
July 2021
Graduate School of Medicine, Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Dispersed single cells from pancreatic islets can configure the three-dimensional islet-like architecture (pseudo-islets) with insulin secretion potential and controllable size through their aggregation property. The present study was designed to investigate whether cryopreservation of islets or islet cells can contribute to the efficient pseudo-islet fabrication in the rat model. In control group (CT), islet single cells were prepared by trypsin digestion of 50-400-µm ø fresh control islets, and then cultured for 3 days in the U-bottom microwell to fabricate pseudo-islets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!