In order to trace the in vivo dynamics of cancer cells, we introduced jellyfish aequorin cDNA into metastatic (A-11) and nonmetastatic (3LL) Lewis lung cancer cell lines. The transformants (e.g., A-11-4 and 3LL-24) remained highly tumorigenic when they were s.c. injected into C57BL/6 mice and the A-11-4 clone produced metastatic tumors in the lung. Luminescence produced by the expressed aequorin was detectable in these transformants in culture as well as in cells of primary or secondary tumors. The limit of detection of the aequorin assay was approximately 90 cells, whereas approximately 5 x 10(4) cells could be macroscopically seen as metastatic foci in the lung. Thus, the aequorin assay is approximately 500-fold more sensitive than macroscopic examination in detecting cancer metastasis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.1997.8045 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Polystyrene Nanoplastics Hitch-Hike the Gut-Brain Axis to Exacerbate Parkinson's Pathology.
ACS Nano
January 2025
Nanomedicine Center, The Great Bay Area National Institute for Nanotechnology Innovation, 136 Kaiyuan Avenue, Guangzhou 510700, China.
The neurological implications of micro- and nanoplastic exposure have recently come under scrutiny due to the environmental prevalence of these synthetic materials. Parkinson's disease (PD) is a major neurological disorder clinically characterized by intracellular Lewy-body inclusions and dopaminergic neuronal death. These pathological hallmarks of PD, according to Braak's hypothesis, are mediated by the afferent propagation of α synuclein (αS) via the enteric nervous system, or the so-called gut-brain axis.
View Article and Find Full Text PDFDual DNA replication modes: varying fork speeds and initiation rates within the spatial replication program in Xenopus.
Nucleic Acids Res
January 2025
Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France.
Large vertebrate genomes duplicate by activating tens of thousands of DNA replication origins, irregularly spaced along the genome. The spatial and temporal regulation of the replication process is not yet fully understood. To investigate the DNA replication dynamics, we developed a methodology called RepliCorr, which uses the spatial correlation between replication patterns observed on stretched single-molecule DNA obtained by either DNA combing or high-throughput optical mapping.
View Article and Find Full Text PDFEarly events in G-quadruplex folding captured by time-resolved small-angle X-ray scattering.
Nucleic Acids Res
January 2025
Department of Medicine, UofL Health Brown Cancer Center, University of Louisville, Louisville KY, 505 S Hancock St, Louisville, KY 40202, United States.
Time-resolved small-angle X-ray experiments are reported here that capture and quantify a previously unknown rapid collapse of the unfolded oligonucleotide as an early step in the folding of hybrid 1 and hybrid 2 telomeric G-quadruplex structures. The rapid collapse, initiated by a pH jump, is characterized by an exponential decrease in the radius of gyration from 24.3 to 12.
View Article and Find Full Text PDFProximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions.
Biol Open
February 2025
Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280, USA.
The network of proteins at the interface between cell-cell adherens junctions and the actomyosin cytoskeleton provides robust yet dynamic connections that facilitate cell shape change and motility. While this was initially thought to be a simple linear connection via classic cadherins and their associated catenins, we now have come to appreciate that many more proteins are involved, providing robustness and mechanosensitivity. Defining the full set of proteins in this network remains a key objective in our field.
View Article and Find Full Text PDFEmerging engineered nanozymes: current status and future perspectives in cancer treatments.
Nanoscale Adv
January 2025
Cancer Center, Department of Interventional Medicine, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College Hangzhou Zhejiang China
Composite nanozymes are composed of enzymes with similar or different catalytic capabilities and have higher catalytic activity than a single enzyme. In recent years, composite nanozymes have emerged as novel nanomaterial platforms for multiple applications in various research fields, where they are used to produce oxygen, consume glutathione, or produce toxic reactive oxygen species (ROS) for cancer therapy. The therapeutic approach using composite nanozymes is known as chemo-dynamic therapy (CDT).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!