AI Article Synopsis
- A deletion occurred in a pUC19 plasmid containing Streptomyces DNA, likely due to a recombination process that doesn't involve the RecA protein.
- The deletion affected both an unknown part of the inserted genomic DNA and a section of the plasmid's multiple cloning site.
- A 13 bp GC-rich sequence, similar to an established hotspot for spontaneous deletions, was discovered at the site of the deletion.
Article Abstract
A spontaneous deletion probably caused by rec A independent recombination between short-direct repeat sequences was observed in pUC19 plasmid carrying a piece of Streptomyces genomic DNA after culturing in liquid medium. The deletion removed an unknown portion of the cloned DNA and the BamHI-EcoRI part of the multiple cloning site with an additional flanking 111 bp from the vector. At the junction a 13 bp GC-rich DNA sequence highly homologous to a known spontaneous deletion hotspot was found.
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