P5abc is a large extension of the P5 element characteristic of subclasses IC1 and IC2 of group I introns. It has a conserved region termed the A-rich bulge, that is responsible for activation of the Tetrahymena self-splicing intron. By employing a modified color-colony assay system, we identified four adenosines in the bulge that are responsible for an efficient splicing reaction. On comparison with the X-ray crystal structure of the P4-5-6 domains of the Tetrahymena intron, three adenosines at positions 183, 184, and 186 were found to be identical to those significantly contributing to the formation of its tertiary structure. However, our results show that an adenosine at 187 is involved in the formation of a Watson-Crick base pair with U135, although it forms a Hoogsteen base pair in the crystal structure.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/oxfordjournals.jbchem.a021836 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Incisions in Hepatobiliopancreatic Surgery: Surgical Anatomy and its Influence to Open and Close the Abdomen.
J Abdom Wall Surg
March 2023
Grupo de Investigación de Pared Abdominal Compleja, Hospital Universitario del Henares, Facultad de Ciencias de la Salud, Universidad Francisco de Vitoria, Madrid, Spain.
Incisions performed for hepato-pancreatic-biliary (HPB) surgery are diverse, and can be a challenge both to perform correctly as well as to be properly closed. The anatomy of the region overlaps muscular layers and has a rich vascular and nervous supply. These structures are fundamental for the correct functionality of the abdominal wall.
View Article and Find Full Text PDFPrenatal ultrasound diagnosis of congenital infantile fibrosarcoma and congenital hemangioma: Three case reports.
World J Clin Cases
October 2023
Department of Ultrasound, The Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
Background: Congenital infantile fibrosarcoma (CIF) and congenital hemangioma (CH) have similarities on prenatal ultrasound and are rare.
Case Summary: We report 3 cases of fetuses with superficial hypervascular tumors, confirmed by postnatal pathology as CIF (1 case) and CH (2 cases, including 1 in a twin fetus). In Case 1, a mass with a rich blood supply in the fetal axilla was discovered by prenatal ultrasound at 28+0 wk of gestation.
Silica-Assisted Controlled Engineering of Nitrogen-Doped Carbon Cages with Bulges for High-Performance Supercapacitors.
ACS Appl Mater Interfaces
December 2021
College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, 70 Yuhua Road, Shijiazhuang 050018, China.
The bulge structure of N-doped carbon cages is beneficial to improving the specific surface area and increasing the active sites of a chemical reaction. Therefore, this structure plays a role in increasing capacity in energy storage. However, the precise and most effective method of ensuring the bulge structures is still a challenge.
View Article and Find Full Text PDFEpithelioid Soft Tissue Neoplasm of the Soft Palate with a PTCH1-GLI1 Fusion: A Case Report and Review of the Literature.
Head Neck Pathol
June 2022
Department of Pathology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.
GLI1 fusions involving ACTB, MALAT1, PTCH1 and FOXO4 genes have been reported in a subset of malignant mesenchymal tumors with a characteristic nested epithelioid morphology and frequent S100 positivity. Typically, these multilobulated tumors consist of uniform epithelioid cells with bland nuclei and are organized into distinct nests and cords with conspicuously rich vasculature. We herein expand earlier findings by reporting a case of a 34-year-old female with an epithelioid mesenchymal tumor of the palate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!