A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC230178 | PMC |
| http://dx.doi.org/10.1128/jcm.35.12.3331-3334.1997 | DOI Listing |
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