AI Article Synopsis
- The trans-Golgi network (TGN) is crucial for forming transport vesicles that send materials to different parts of the cell.
- TGN38/46 is a significant protein marker for the mammalian TGN, involved in recruiting factors for vesicle formation.
- This study discovered that a specific enzyme related to a type of lipid influences the development of secretory vesicles, showing similarities between yeast and mammalian cells.
Article Abstract
The eukaryotic trans-Golgi network (TGN) is a key site for the formation of transport vesicles destined for different intracellular compartments [1]. A key marker for the mammalian TGN is TGN38/46 [2]. This integral membrane glycoprotein cycles between the TGN and the cell surface and is implicated in recruitment of cytosolic factors and regulation of at least one type of vesicle formation at the mammalian TGN [2] [3]. In this study, we have identified a phosphatidylinositol (PtdIns)-specific 3-kinase activity associated with the human orthologue (TGN46), which is sensitive to lipid kinase inhibitors. Treatment of HeLa cells with low levels of these inhibitors reveals subtle morphological changes in TGN46-positive compartments. Our findings suggest a role for PtdIns 3-kinases and presumably for the product, PtdIns 3-phosphate (PtdIns3P), in the formation of secretory transport vesicles by mechanisms conserved in yeast and mammals.
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| http://dx.doi.org/10.1016/s0960-9822(06)00418-0 | DOI Listing |
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