Using statistical and morphometric methods, the authors validate a new method for assessing the quantity of spermatozoa per ml of native ejaculate in a fixed and stained smear. The proposed method is regarded as an alternative to the routine technique.
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CRISPR-Cas-Based Pen-Side Diagnostic Tests for and .
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Health Program, International Livestock Research Institute (ILRI), Nairobi P.O. Box 30709, Kenya.
and are tick-borne pathogens, posing significant threats to the health and productivity of cattle in tropical and subtropical regions worldwide. Currently, detection of and in infected animals relies primarily on microscopic examination of Giemsa-stained blood or organ smears, which has limited sensitivity. Molecular methods offer higher sensitivity but are costly and impractical in resource-limited settings.
View Article and Find Full Text PDFA Retrospective Epidemiological Analysis of Microscopically Detected Babesiosis in Dogs of Southern Poland (2018-2022).
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December 2024
Department of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland.
is the parasite responsible for a life-threatening disease for dogs in Central Europe, of which the main vector is the ornate dog tick-. The objective of the presented study was to assess the prevalence of infection in dogs with clinical suspicion of babesiosis, which tested positive for from locations where there is no or very limited information about dog exposure to this pathogen. In order to confirm the presence of this protozoan, blood samples were collected from dogs treated in veterinary clinics with suspicion of canine babesiosis.
View Article and Find Full Text PDFInvestigating Endoparasites in Captive Birds of Prey in Italy.
Animals (Basel)
December 2024
Department of Veterinary Medicine and Animal Sciences, Università Degli Studi di Milano, Via Dell' Università 6, 26900 Lodi, Italy.
Birds of prey can be parasitised by several endoparasites that can coexist without clinical signs of disease or occur in conjunction with stressful events. Because the number of birds of prey kept in captivity is copiously increasing due to their use for bird control, breeding programs, exhibition and falconry, the main endoparasites of 81 apparently healthy captive birds of prey from northern Italy were investigated by examining faecal and blood samples. Faeces were analysed by a quali-quantitative technique, i.
View Article and Find Full Text PDFEvaluation of an enhanced ResNet-18 classification model for rapid On-site diagnosis in respiratory cytology.
BMC Cancer
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Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China.
Objective: Rapid on-site evaluation (ROSE) of respiratory cytology specimens is a critical technique for accurate and timely diagnosis of lung cancer. However, in China, limited familiarity with the Diff-Quik staining method and a shortage of trained cytopathologists hamper utilization of ROSE. Therefore, developing an improved deep learning model to assist clinicians in promptly and accurately evaluating Diff-Quik stained cytology samples during ROSE has important clinical value.
View Article and Find Full Text PDFThyroid cytopathology, particularly in cases of atypia of undetermined significance/follicular lesions of undetermined significance (AUS/FLUS), suffers from suboptimal sensitivity and specificity challenges. Recent advancements in digital pathology and artificial intelligence (AI) hold promise for enhancing diagnostic accuracy. This systematic review included studies from 2000 to 2023, focusing on diagnostic accuracy in AUS/FLUS cases using AI, whole slide imaging (WSI), or both.
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