Overwintering status and cold hardiness of hypera punctata.

We investigated overwintering status and various characteristics related to cold hardiness in the clover leaf weevil, Hypera punctata. The larvae continue to feed and grow in winter. The proportion of late instar larvae in a field population increased gradually between December and the following March, and fourth (last) instar larvae were found in mid-February onward. The lower thermal threshold of development was lower in the larval stage than in the other developmental stages. The supercooling point (SCP) varied with developmental stages. There was a close positive correlation between the SCP and larval body weight. Deprivation of food for 3 days increased the correlation and decrease their SCP by 5 to 8 degrees C in larvae at any instar. The SCP of overwintering larvae did not differ from that of fed larvae in the laboratory. The food-deprived early instar larvae in the laboratory died without being frozen, while the lower lethal temperature of fed larvae in the laboratory and overwintering larvae were almost equivalent to the respective SCPs at a temperature normally encountered in the field. These suggested that this weevil may not increase their cold hardiness during winter so far and the SCP may serve as an indicator of cold hardiness. Copyright 1997 Academic Press. Copyright 1997Academic Press