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PathoGD: an integrative genomics approach to primer and guide RNA design for CRISPR-based diagnostics.
Commun Biol
January 2025
Department of Infectious Diseases, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
Critical to the success of CRISPR-based diagnostic assays is the selection of a diagnostic target highly specific to the organism of interest, a process often requiring iterative cycles of manual selection, optimisation, and redesign. Here we present PathoGD, a bioinformatic pipeline for rapid and high-throughput design of RPA primers and gRNAs for CRISPR-Cas12a-based pathogen detection. PathoGD is fully automated, leverages publicly available sequences and is scalable to large datasets, allowing rapid continuous monitoring and validation of primer/gRNA sets to ensure ongoing assay relevance.
View Article and Find Full Text PDFFirst report of causing leaf spot of (Magnoliaceae) in China.
Plant Dis
January 2025
Huainan Normal University, School of Bioengineering, Dongshan West Road, Huainan City, Huainan, China, 232038;
Manglietia decidua is an extremely endangered species, known for its limited population and a narrow distribution range restricted to China (Yu 1994). In October 2021, a leaf disease affecting the foliage of 3-year-old M. decidua was observed at the nursery garden of the Yichun Forestry Institute of Jiangxi Province (27°55'52.
View Article and Find Full Text PDFFirst report of leaf spot disease caused by on L. in Heilongjiang Province, China.
Plant Dis
January 2025
600 Changjiang Road, HarbinHarbin, China, 150030;
'Lanjingling' [China National Plant Variety Protection (CNPVP) 20200389] is the first new nationally registered cultivar of blue honeysuckle (Lonicera caerulea L.) developed by the Northeast Agricultural University for the fresh-fruit market (Zhu et al. 2022).
View Article and Find Full Text PDFLogical Analysis of Multiple miRNAs with Isothermal Molecular Classifiers Based on LATE-RCA.
Nano Lett
January 2025
Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P. R. China.
Logical analysis of multiple-miRNA expression information and immediate output of diagnostic results facilitates early cancer detection. In this work, we constructed an isothermal molecular classifier capable of performing computations on multiple miRNAs and directly providing diagnosis results. First, we developed linear-after-the-exponential rolling circle amplification (LATE-RCA), a nearly linear isothermal amplification that does not destroy the original quantitative information about miRNAs.
View Article and Find Full Text PDFUnderstanding the vaginal microbiome among women with different genotypes of human papillomavirus infection in remote Andaman islands.
Front Cell Infect Microbiol
January 2025
Indian Council of Medical Research - Regional Medical Research Centre, Port Blair, Andaman and Nicobar Islands, India.
Background: Human papillomavirus (HPV) is a viral infection, and its acquisition and persistence are significantly influenced by the vaginal microbiota. Understanding and comparing the vaginal microbiome of HPV infected women in Andaman and Nicobar Islands is crucial.
Methods: The study involved collecting vaginal swabs and extracting DNA using the QIAamp DNA Minikit.
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