For the purposes of the antisense strategy oligodeoxyribonucleotides can be protected against serum and cell nuclease digestion by tagging at their 3'-end with a sequence naturally forming a very stable hairpin, d(GCGAAGC). This nuclease-resistant hairpin is also known for its high thermostability. We demonstrate in this study that attachment of d(GCGAAGC) at the 3'-end of an oligodeoxyribonucleotide does not hinder hybridization of the 5'-part of this oligonucleotide to a complementary DNA strand. Moreover, the hairpin is in equilibrium between a folded and an open structure, with an energy minimum in favor of pairing if it is possible, even with mismatches.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC147075 | PMC |
| http://dx.doi.org/10.1093/nar/25.22.4608 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Old innovations and shifted paradigms in cellular neuroscience.
Front Cell Neurosci
August 2024
Department of Biomedical Sciences, Humanitas University Medical School, Pieve Emanuele, Italy.
Once upon a time the statistics of quantal release were fashionable: "" available vesicles (fusion sites), each with probability "" of releasing a quantum. The story was not so simple, a nice paradigm to be abandoned. Biophysicists, experimenting with "black films," explained the astonishing rapidity of spike-induced release: calcium can trigger the fusion of lipidic vesicles with a lipid bilayer, by masking the negative charges of the membranes.
View Article and Find Full Text PDFHuman NK cells and cancer.
Oncoimmunology
July 2024
Tumor Immunology Unit, Bambino Gesù Children's Hospital IRCCS, Rome, Italy.
The long story of NK cells started about 50 y ago with the first demonstration of a natural cytotoxic activity within an undefined subset of circulating leukocytes, has involved an ever-growing number of researchers, fascinated by the apparently easy-to-reach aim of getting a "universal anti-tumor immune tool". In fact, in spite of the impressive progress obtained in the first decades, these cells proved far more complex than expected and, paradoxically, the accumulating findings have continuously moved forward the attainment of a complete control of their function for immunotherapy. The refined studies of these latter years have indicated that NK cells can epigenetically calibrate their functional potential, in response to specific environmental contexts, giving rise to extraordinarily variegated subpopulations, comprehensive of memory-like cells, tissue-resident cells, or cells in various differentiation stages, or distinct functional states.
View Article and Find Full Text PDFCan Re cluster complexes be an efficient catalyst for hydrogen evolution reaction? Insights from experiments and computations.
Dalton Trans
May 2024
Kazan (Volga region) Federal University, 18, Kremlyovskaya str., 420008 Kazan, Russian Federation.
To date, researchers in chase of economic cost-efficiency are faced with the problem of developing effective catalysts for water splitting without the use of platinoids. Herein, catalytic properties of hexanuclear rhenium cluster complexes are investigated in application to the hydrogen evolution reaction (HER). A paste composite electrode containing the cluster complexes was obtained, producing a current density of 10 mA cm at an extraordinarily low overpotential of 90 mV (RHE).
View Article and Find Full Text PDFMicrogram BaCl2 ablation targets for trapped ion experiments.
Rev Sci Instrum
April 2024
Institute for Quantum Computing and Department of Physics and Astronomy, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Trapped ions for quantum information processing have been an area of intense study due to the extraordinarily high fidelity operations that have been reported experimentally. Specifically, barium trapped ions have been shown to have exceptional state-preparation and measurement fidelities. The 133Ba+ (I = 1/2) isotope in particular is a promising candidate for large-scale quantum computing experiments.
View Article and Find Full Text PDFHCOO Doping-Induced Multiexciton Emissions in CsCuI Crystals for Efficient X-Ray Scintillation.
Small
August 2024
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, China.
Self-trapped exciton (STE) luminescence, typically associated with structural deformation of excited states, has attracted significant attention in metal halide materials recently. However, the mechanism of multiexciton STE emissions in certain metal halide crystals remains largely unexplored. This study investigates dual luminescence emissions in HCOO doped CsCuI single crystals using transient and steady-state spectroscopy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!