To improve identification of M. bovis, rabbit immune sera and mouse monoclonal antibodies against M. bovis-secreted protein antigens were used in the enzyme-linked assay (ELISA). In western blot analysis, M. bovis-specific epitopes within culture filtrate proteins were demonstrated to be mostly concentrated on the immunodominant 35-38 kDa antigen. Polyclonal and cross-reactive monoclonal antibodies were shown to be able to bind to all mycobacterial strains tested in ELISA (M. tuberculosis, M. bovis, M. kansasii, M. marinum, M. avium, M. smegmatis), but not to bacteria of the other genera. Among the monoclonal antibodies against M. bovis specific antigen, 2-6B was found to be the only one which could evidently react with whole cells of M. bovis in ELISA, but not with those of the other mycobacteria including M. tuberculosis.
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