Regulatory differences among avian ecto-ATPases.

Biochem Biophys Res Commun

Department of Chemistry, Molecular Biology Institute, San Diego State University, California 92182, USA.

Published: September 1997

Extracellular ATP is found to produce a variety of important biological responses. Ecto-ATPases are located on numerous cell types in many different species, regulate extracellular ATP levels and can be a key step in generating adenosine. Studies conducted on chicken ecto-ATPases from liver and cardiac and smooth muscle show a variety of differing properties including (1) different apparent Km's, (2) lectin sensitivity, (3) responses to detergents, (4) responses to lipid mediators, and (5) responsiveness to nucleotide-mimetic affinity labels. These results suggest that although each enzyme hydrolyzes extracellular ATP, they should each be viewed as a distinct subtype of the whole ecto-ATPase family due to their differential responses, largely linked to proposed regulatory phenomenon.

Download full-text PDF

Source
http://dx.doi.org/10.1006/bbrc.1997.7377DOI Listing

Publication Analysis

Top Keywords

extracellular atp
12
regulatory differences
4
differences avian
4
avian ecto-atpases
4
ecto-atpases extracellular
4
atp produce
4
produce variety
4
variety biological
4
responses
4
biological responses
4

Similar Publications

Unlabelled: During infection, bacterial pathogens rely on secreted virulence factors to manipulate the host cell. However, in gram-positive bacteria, the molecular mechanisms underlying the folding and activity of these virulence factors after membrane translocation are not clear. Here, we solved the protein structures of two secreted parvulin and two secreted cyclophilin-like peptidyl-prolyl isomerase (PPIase) ATP-independent chaperones found in gram-positive streptococcal species.

View Article and Find Full Text PDF

Fluorofurimazine, a novel NanoLuc substrate, enhances real-time tracking of influenza A virus infection without altering pathogenicity in mice.

Microbiol Spectr

January 2025

Department of Microbiology and Immunology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, Alabama, USA.

Unlabelled: Bioluminescence imaging (BLI) using engineered bioluminescent viruses has emerged as a powerful tool for real-time, noninvasive monitoring of viral replication in living animals. While traditional luciferase-based systems, such as firefly luciferase, have been widely used, the NanoLuc luciferase system offers distinct advantages, including its significantly smaller gene size, increased brightness, and independence from ATP as a cofactor, allowing for extracellular detection. However, the utility of NanoLuc has been limited by its traditional substrate, furimazine, which exhibits poor water solubility and potential cytotoxicity.

View Article and Find Full Text PDF

The ABCB4 gene encodes multidrug resistance protein 3(MDR3), which is a phosphatidylcholine(PC) transfer enzyme that transfers lecithin from the inner part of the phospholipid bilayer to the extracellular bile. The occurrence of intrahepatic cholestasis of pregnancy(ICP) is closely related to ABCB4 variants, but there is limited research on this topic in southern Anhui, China. We sequenced ABCB4 in pregnant women with ICP and healthy pregnant women to explore the relationship.

View Article and Find Full Text PDF

Metagenomic insight into the diffusion signal factor mediated social traits of anammox consortia after starvation.

J Environ Manage

January 2025

School of Engineering, Hangzhou Normal University, Hangzhou, 311121, China; School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China. Electronic address:

Biomass starvation is common in biological wastewater treatment. As a social trait of microbial community, how quorum sensing (QS) regulated bacterial trade-off through interactions after starvation remains unclear. This study deciphered the mechanism of anaerobic ammonium oxidation (anammox) consortia in response to starvation, including reducing extracellular electron transfer (EET), adenosine 5'-triphosphate (ATP) content and amino acid metabolism.

View Article and Find Full Text PDF

Plants deploy cellular Ca2+ elevation as a signal for environmental stress signaling. Extracellular ATP (eATP) is released into the extracellular matrix when cells are wounded. DOES NOT RESPOND TO NUCLEOTIDES 1 (DORN1), a key legume-type lectin receptor, senses and binds eATP and activates Ca2+ signaling.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!