In 48 h incubation L-929 (L-5) cells secreted collagenous protein accounting for 16% of the total protein. CM-chromatography, SDS-acrylamide gel electrophoresis and DEAE-cellulose chromatography revealed that the collagenous protein was mainly composed of type I collagen, having no collagen of type III. In addition, precursor-form collagen and tropocollagen molecules were found to be present in the incubate.
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| http://dx.doi.org/10.1007/BF00558009 | DOI Listing |
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