The fractional composition of the vaccine preparation obtained by the hydroxylamine treatment of K. pneumoniae 204 cells was studied with the use of gel chromatography in sepharose CL-6B. The preparation was shown to form 2 peaks, only the first high molecular peak being serologically active. The method for the treatment of the culture, obtained directly from a fermenter and stored in a frozen state before treatment, was proposed. In the technology of the manufacture of Klebsiella vaccine preparation the possibility of replacing dialysis with gel chromatography was shown.
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