Both free and hidden natural antibodies to DNA or cardiolipin were obtained from immunoglobulins of a normal donor. The free antibodies reacting with DNA or cardiolipin were isolated by means of affinity chromatography. Antibodies occurring in an hidden state were disengaged from the depleted immunoglobulins by ion-exchange chromatography and were then affinity-isolated on DNA or cardiolipin sorbents. We used flow cytometry to study the ability of free and hidden antibodies to bind to rat thymocytes. Simultaneously, plasma membrane integrity was tested by propidium iodide (PI) exclusion. The hidden antibodies reacted with 65.2 +/- 10.9% of the thymocytes and caused a fast plasma membrane disruption. Cells (28.7 +/- 7.1%) were stained with PI after incubation with the hidden antibodies for 1 h. The free antibodies bound to a very small fraction of the thymocytes and did not evoke death as compared to control without antibodies. The possible reason for the observed effects is difference in reactivity of the free and hidden antibodies to phospholipids. While free antibodies reacted preferentially with phosphotidylcholine, hidden antibodies reacted with cardiolipin and phosphotidylserine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0014-5793(97)00843-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.
Vet Med Sci
January 2025
Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Background: Brucellosis is a zoonotic disease caused by Brucella spp., affecting various animals and humans, leading to significant economic and public health impacts. Traditional diagnostic methods, mainly serological, often fail to detect seronegative carriers, which continue to spread the infection.
View Article and Find Full Text PDFUse of a hidden Markov model for interpretation of serial cow milk paratuberculosis antibody enzyme-linked immunosorbent assay results adjusted for milk yield and quality.
Prev Vet Med
February 2025
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, United Kingdom.
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a common, economically-important and potentially zoonotic contagious disease of cattle, with worldwide distribution. Disease management relies on identification of animals which are at high-risk of being infected or infectious.
View Article and Find Full Text PDFReactivation of hidden-latent infection after doxycycline and streptomycin treatment in mice.
Antimicrob Agents Chemother
December 2024
Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Brucellosis has therapeutic challenges due to 3%-15% relapses/therapeutic failures (R/TF) after antibiotic treatment. Therefore, determining the antibiotic concentration in tissues, the physiopathological parameters, and the R/TF after treatment is relevant. After exploring different antibiotic quantities, we found that a combined dose of 100 µg/g of doxycycline (for 45 days) and 7.
View Article and Find Full Text PDFAnti-amyloid treatments in Alzheimer's disease: elegance, evidence and ethics.
Adv Clin Exp Med
December 2024
NeuroActiva, Inc., San Jose, USA.
The so-called "amyloid cascade hypothesis" provides an elegant explanation of Alzheimer's disease (AD), has motivated the amyloid-lowering therapeutic strategy, and led to the elaboration of a rich experimental and conceptual toolkit for the field to progress. But it might be incorrect. The scientific evidence base supporting the efficacy and safety of current anti-amyloid antibody treatments in AD is weak.
View Article and Find Full Text PDFA novel shark VNAR antibody-based immunotoxin targeting TROP-2 for cancer therapy.
Acta Pharm Sin B
November 2024
College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Article Synopsis
- - TROP-2, a protein linked to various types of cancer, is a potential target for new cancer treatments, specifically antibody-drug conjugates (ADCs), but their effectiveness against solid tumors is limited due to issues like poor penetration.
- - Researchers developed a small, stable immunotoxin using a shark-derived antibody known as VNAR, which has better tissue penetration properties than traditional antibodies.
- - The study identified a specific VNAR, called VNAR-5G8, which binds effectively to TROP-2 and created a recombinant immunotoxin (5G8-PE38) that showed strong anti-tumor activity, suggesting its potential as a cancer therapy option.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!