We present the genotyping of apolipoprotein (apo) E by means of restriction fragment analysis of amplified genomic DNA by high-performance capillary electrophoresis and a replaceable non-gel-sieving matrix. This procedure streamlines the genotyping of apo E in large-scale population studies because of the automation and speed of capillary electrophoresis.
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Profiles Drug Subst Excip Relat Methodol
January 2025
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia. Electronic address:
Nateglinide belongs to the meglitinide class of insulin secretagogues. It is used as an oral hypoglycemic agent for the treatment of type 2 diabetes mellitus. Nateglinide is an amino acid derivative of D-phenylalanine that binds to the ATP-sensitive potassium channels in pancreatic beta cells and stimulates the secretion of insulin.
View Article and Find Full Text PDFFood Res Int
February 2025
Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo, Ningbo 315100, China. Electronic address:
Xylooligosaccharides (XOS), short-chain polymers with prebiotic properties, have gained significant commercial attention over the past few decades due to their potential as nutraceutical components. Derived from lignocellulosic biomass (LCB), XOS serve as health promoting compounds with applications across multiple sectors, including food pharmaceutical and cosmetic. This comprehensive review provides an overview of XOS production, purification, characterization, and quantification, highlighting their derivation from various sources such as agricultural waste, agro-economical forest residues, and nutrient-dense energy crops.
View Article and Find Full Text PDFAnnu Rev Anal Chem (Palo Alto Calif)
January 2025
Department of Chemistry, Michigan State University, East Lansing, Michigan, USA; email:
Mass spectrometry (MS)-based top-down proteomics (TDP) characterizes proteoforms in cells, tissues, and biological fluids (e.g., human plasma) to better our understanding of protein function and to discover new protein biomarkers for disease diagnosis and therapeutic development.
View Article and Find Full Text PDFJ Transl Med
January 2025
Dipartimento di Pediatria Generale e d'Urgenza, AORN Santobono-Pausilipon, Naples, Italy.
Background: Multisystem inflammatory syndrome in children (MIS-C) is a severe post-COVID condition due to a delayed hyperimmune response to SARS-CoV-2. High-density lipoproteins (HDL) are pivotal players in inflammatory and immune modulation through the remodeling of their subfractions.
Methods: This study aimed to evaluate serum levels of cholesterol, HDL, and HDL subfractions (HDL-SUB) to define their role in the pathogenesis of MIS-C and their potential use as biomarkers of this condition.
Talanta
January 2025
DSM-Firmenich, Kogle Allé 4, 2970, Hørsholm, Denmark.
The development and validation of an accurate, selective, and eco-friendly capillary zone electrophoretic detection (CZE) method has been presented for concurrent measurement of inorganic and organic anions including chloride, sulfate, formic acid, citric acid, acetic acid, phosphate, and glutamic acid in Human Milk Oligosaccharides (HMOs) for the first time. An electrolyte composed of an aqueous solution of benzoic acid, 16.38 mM; l-histidine, 24.
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