When added to the suspension of washed cells of Tolypocladium inflatum (cyclosporine-producing organism) in a concentration up to 100 micrograms/ml, cyclosporine A had no effect on permeability of the cell covers as judged by the quantity of the metabolites with the absorption maxima at 260 nm that leaked to the medium. When cyclosporine was added to the suspension of the T-inflatum cell protoplasts there was a significant increase in the metabolites leaking. Impairment of the permeability of the protoplast membranes was more marked in low potent strains. Impairment of the protoplast membrane permeability by amphotericin B was equal in both the high and the low potent strains and in this respect it was more active than cyclosporine.
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