Comparison of three commercial media for direct identification and discrimination of Candida species in clinical specimens.

Eur J Clin Microbiol Infect Dis

Laboratoire de Bactériologie, Hôpital de l'Antiquaille, Lyon, France.

Published: June 1997

AI Article Synopsis

  • - A study compared three types of chromogenic media (Albicans ID, Candiselect, and CHROMagar Candida) with a standard method (Sabouraud-chloramphenicol agar) for identifying yeasts using 192 clinical specimens.
  • - Detection rates for the media were relatively close, with the chromogenic media showing specific identification rates for Candida albicans increasing significantly from 24 to 72 hours of incubation.
  • - CHROMagar Candida successfully identified additional strains of Candida tropicalis and Candida krusei after 48 hours, demonstrating high specificity (100%) for the tested strains.

Article Abstract

One hundred and ninety-two clinical specimens were used to compare the three chromogenic media Albicans ID, Candiselect, and CHROMagar Candida to a standard method using a Sabouraud-chloramphenicol agar medium and standard methods for identification of yeasts. The detection rates were 83.79, 83.24, 86.59 and 84.91% respectively. For the chromogenic media, the rates of direct identification (growth plus specific pigmentation) for Candida albicans were 56.50, 37.68 and 11.59% after 24 hours' incubation and 92.75, 91.30 and 88.57% after 72 hours' incubation respectively, with 100% specificity. Furthermore, CHROMagar Candida identified the five Candida tropicalis and the two Candida krusei strains detected after 48 hours' incubation.

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http://dx.doi.org/10.1007/BF02471913DOI Listing

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