Postmitochondrial supernatant, cytosol, and microsomes were exposed to near-ultraviolet light and assayed for incorporation of (3H)leucine. The results indicate that near-ultraviolet light decreased the incorporation of radioactivity into the aminoacyl-tRNA fraction and into proteins. This effect depended on the wavelength of irradiation and the concentration of exposed protein. Tryptophan and epinephrine potentiated the effect of near-ultraviolet light, but catalase prevented this effect. Hydrogen peroxide decreased protein synthesis when it was added to unirradiated postmitochondrial supernatant. The results suggest that near-ultraviolet light would decrease protein synthesis by generating hydrogen peroxide from tryptophan and epinephrine.
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Small Methods
January 2025
Institute of New Energy Technology, College of Physics & Optoelectronic Engineering, Jinan University, Guangzhou, Guangdong, 510632, China.
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January 2025
Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, China.
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Faculty of Materials Science and Engineering, Phenikaa University Yen Nghia, Ha-Dong District Hanoi 10000 Vietnam
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Northwest Institute for Nonferrous Metal Research, Xi'an 710016, China.
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Department of Physics, RTM Nagpur University, Nagpur 440033, India.
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