Sensitivity of Staphylococcus aureus to the antibiotics tetracycline, benzylpenicillin and vancomycin was found to decrease by 2-10 fold when cells were grown adherent to silicone catheter surfaces. Sensitivity to rifampicin and fusidic acid was not significantly altered in adherent cells. Susceptibility further decreased with increased adherence time prior to antibiotic challenge. The resistance observed was not genotypic, or due to the presence of a specialized subpopulation of bacteria, as it disappeared when the bacteria were removed from the catheter, subcultured and retested. Also, adherent bacteria were found to grow more slowly than bacteria growing planktonically. It is concluded that the decrease in antibiotic susceptibility of adherent bacteria is a function of the physiological status of the individual cells rather than a function of biofilm formation or slime production. The decrease in growth rate of the adherent bacteria is a result of the adherence process rather than a result of nutrient depletion. The decrease in growth rate is implicated, but is not the sole factor, in the decreased antibiotic susceptibility of adherent bacteria.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/00221287-143-7-2407 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative Effects of Antibiotic and Antimicrobial Peptide on Growth Performance, Gut Morphology, Intestinal Lesion Score, Ileal Microbial Counts, and Immune Status in Broilers Challenged with Necrotic Enteritis.
Probiotics Antimicrob Proteins
January 2025
Department of Poultry Production, Faculty of Animal Production and Technology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
This experiment aimed to compare the efficacy of an antimicrobial peptide (AMP) with a conventional antibiotic growth promoter (AGP) during necrotic enteritis (NE) challenge in broilers. In total, 720 1-day-old exclusively male broiler chicks (Ross-308) were allocated to five treatments, each with six replicates of 24 birds (n = 144/treatment), for 35 days. The treatments were as follows: (1) uninfected control (UC) with basal diet, (2) infected control (IC) with C.
View Article and Find Full Text PDFSex hormone-dependent host-microbiome interactions and cardiovascular risk (XCVD): design of a longitudinal multi-omics cohort study.
BMJ Open
January 2025
Experimental and Clinical Research Center, Charité - Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Introduction: Cardiovascular diseases (CVDs) present differently in women and men, influenced by host-microbiome interactions. The roles of sex hormones in CVD outcomes and gut microbiome in modifying these effects are poorly understood. The XCVD study examines gut microbiome mediation of sex hormone effects on CVD risk markers by observing transgender participants undergoing gender-affirming hormone therapy (GAHT), with findings expected to extrapolate to cisgender populations.
View Article and Find Full Text PDFDuration of antibiotic therapy in the intensive care unit: factors influencing decision-making during multidisciplinary meetings.
BMJ Qual Saf
January 2025
IQ Health Science Department, Radboud university medical center, Nijmegen, Gelderland, Netherlands.
Objectives: In the intensive care unit (ICU), antibiotics are often given longer than recommended in guidelines. A better understanding of the factors influencing antibiotic therapy duration is needed to develop improvement strategies to effectively address these drivers of excessive duration. This study aimed to explore the determinants of adherence to recommended antibiotic therapy durations among healthcare professionals involved in antibiotic decision-making within the ICU, focusing on multidisciplinary meetings (MDMs).
View Article and Find Full Text PDFLinking E. coli to fibrosis in Crohn's disease.
Cell Host Microbe
January 2025
The Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland; UCD School of Medicine, University College Dublin, Dublin, Ireland. Electronic address:
Intestinal fibrosis associated with Crohn's disease is a serious yet poorly understood clinical complication. In this issue of Cell Host & Microbe, Ahn and colleagues provide evidence that the adherent intestinal E. coli produced the metallophore yersiniabactin, which sequesters zinc to drive intestinal fibrosis in a HIF-1α-dependent manner.
View Article and Find Full Text PDFHousehold transmission of SARS-CoV-2 in five US jurisdictions: Comparison of Delta and Omicron variants.
PLoS One
January 2025
COVID-19 Response Team, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
Households are a significant source of SARS-CoV-2 transmission, even during periods of low community-level spread. Comparing household transmission rates by SARS-CoV-2 variant may provide relevant information about current risks and prevention strategies. This investigation aimed to estimate differences in household transmission risk comparing the SARS-CoV-2 Delta and Omicron variants using data from contact tracing and interviews conducted from November 2021 through February 2022 in five U.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!