AI Article Synopsis
- Rapid virus detection methods, like RT-PCR followed by sequencing, are crucial for studying outbreaks.
- A study compared RT-PCR with traditional cell culture methods to detect louping ill virus in ticks and found strong correlation between results.
- This efficient system enhances the ability to conduct thorough studies on the epidemiology of louping ill virus.
Article Abstract
Rapid and precise virus detection procedures are an important component of any epizootiological study. An automated one tube reverse transcriptase and nested primer polymerase chain reaction (RT-PCR) followed by nucleotide sequencing of the cDNA product, was used for the rapid detection and identification of louping ill (LI) virus in field caught Ixodes ricinus and compared with a classical isolation method i.e. infectivity in cell culture. The results establish the genetic identity of LI virus on the Lochindorb Estate. There was a high correlation between the results obtained by RT-PCR and infectivity assays. RT-PCR and sequencing proved to be a rapid and accurate system for identifying LI virus in field specimens. Development of this system should improve the capacity to undertake detailed epizootiological studies of LI virus.
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| http://dx.doi.org/10.1007/s007050050151 | DOI Listing |
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