AI Article Synopsis
- Researchers purified porphyrinogen carboxylyase from normal rat liver using two different protocols involving liver homogenate, centrifugation, salt precipitation, and phosphate gel absorption.
- Scheme I utilized three types of column chromatography (DEAE-cellulose, Sephacryl S-200, and Phenyl-sepharose), while Scheme II used an affinity column followed by Sephadex G-75 gel filtration.
- The findings indicated that the enzyme's activity and stability weren't improved with dithiothreitol, and different purification methods yielded varying enzyme activities based on the substrate used, suggesting the presence of at least two isoenzymes.
Article Abstract
Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase.
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Porphyria-induced hepatic porphyrinogen carboxy-lyase inhibitor and its interaction with the active site(s) of the enzyme.
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