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Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits.
J Transl Med
January 2025
Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar.
Background: FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. This study compared the effectiveness of seven different commercially available RNA extraction kits specifically designed for use with FFPE samples in terms of the quantity and quality of RNA recovered.
Methods: This study used 9 samples of FFPE tissue from three different types of tissue (Tonsil, Appendix and lymph node of B-cell lymphoma) to evaluate RNA extraction methods.
Drivers of HIV self-test kit among Tanzanian men aged 15-49: findings from the 2022 TDHS-MIS cross-sectional study.
AIDS Res Ther
January 2025
Department of Mathematics, Physics and Informatics, Mkwawa University College of Education, Iringa, Tanzania.
Introduction: The introduction of the HIV self-test kit in the early 2000s was a major breakthrough in combating HIV. This study determines the social demographic and sexual behaviour driving the use of HIV self-test kits.
Method: The study used secondary data obtained from Tanzania DHS-MIS 2022.
Introductory Analysis and Validation of CUT&RUN Sequencing Data.
J Vis Exp
December 2024
Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center; Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center;
The CUT&RUN technique facilitates detection of protein-DNA interactions across the genome. Typical applications of CUT&RUN include profiling changes in histone tail modifications or mapping transcription factor chromatin occupancy. Widespread adoption of CUT&RUN is driven, in part, by technical advantages over conventional ChIP-seq that include lower cell input requirements, lower sequencing depth requirements, and increased sensitivity with reduced background signal due to a lack of cross-linking agents that otherwise mask antibody epitopes.
View Article and Find Full Text PDFIL-17 affects the immune regulation of CD4+ T cells in dilated cardiomyopathy through JAK/STAT pathway.
Cardiol Young
January 2025
Department of Cardiovascular, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Article Synopsis
- The study investigates how interleukin-17 (IL-17) affects CD4+ T-cell immune regulation and the JAK/STAT signaling pathway, potentially leading to new therapies for dilated cardiomyopathy.
- Naive CD4+ T cells from mice were manipulated to either overexpress or knockdown IL-17, with multiple proteins measured via methods like Western blotting and PCR to assess inflammatory responses and signaling pathways.
- Results showed that overexpressing IL-17 increased levels of inflammatory factors and apoptosis in CD4+ T cells, while knocking it down decreased these levels, highlighting IL-17's significant regulatory role in inflammation and immune response.
[Clinical Impact of Current Variants in COVID-19 Patients: Clinical Characteristics in Variant EG.5].
Mikrobiyol Bul
October 2024
University of Health Sciences, Ankara Bilkent City Health Application and Research Center, Clinic of Medical Microbiology, Ankara, Türkiye.
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has mutated at a high rate since the beginning of the pandemic, leading to the formation of different variants. Alpha, Beta, Gamma, Delta and Omicron have emerged as concerning variants identified by the World Health Organization (WHO). The Omicron variant and its sublineages became dominant worldwide in 2022.
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