NASBA, an isothermal amplification method for nucleic acids, was applied to the detection of RNA of potato leafroll virus (PLRV) in a single enzymatic reaction at 41 degrees C. A set of primers was selected from the coat protein open reading frame sequence of PLRV to allow amplification of viral RNA. The NASBA reaction products were visualized after electrophoresis by ethidium bromide or acridine orange staining. The specificity of the amplification products was validated by Northern blot analysis with a PLRV-specific 32P-labelled oligonucleotide probe. The procedure was coupled to immunocapture of PLRV virions from tuber extracts by immobilized antibodies in microtubes. It was possible to discriminate readily by this method between uninfected and primarily PLRV-infected potato tubers. NASBA is suitable for the direct detection of PLRV in potato tubers from primarily infected plants, offering the potential to considerably simplify the inspection of seed-potatoes for virus infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0166-0934(97)02203-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The effect of planting density on producing quality seed tubers using shoot tip cuttings and conventional methods from tubers has not been studied in Ethiopia. An experiment was conducted to determine the effects of spacing on seed tuber yield and related traits of potato cultivars at Adet Agricultural Research Center in northwestern Ethiopia during the 2023 cropping season. The treatments consisted of two potato varieties (Belete and Gera) propagated by shoot tip cuttings at four inter-row spacings (30, 40, 50, and 60 cm) and intra-row spacing (15, 20, 25, and 30 cm).
View Article and Find Full Text PDFManipulating stomatal aperture by silencing StSLAC1 affects potato plant-herbivore-parasitoid tritrophic interactions under drought stress.
New Phytol
January 2025
State Key Laboratory of Rice Biology, Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China.
The effects of drought stress on stomatal opening dynamics, plant volatile organic compound (VOC) emissions and plant-insect interactions have been well-documented individually, but how they interact mechanistically remains poorly studied. Here, we studied how drought-triggered stomatal closure affects VOC emission and plant-trophic interactions by combining RNAi silencing, molecular biological and chemical analyses (GC-MS) of a potato-tuber moth-egg parasitoid tritrophic system. Drought stress attenuated stomatal apertures and VOC emissions, which made the potato (Solanum tuberosum L.
View Article and Find Full Text PDFThe Induction of Disease Resistance by Scopolamine and the Application of Extract Against Potato ( L.) Late Blight.
Int J Mol Sci
December 2024
School of Life Sciences, Chongqing University, Chongqing 401331, China.
Late blight, caused by , is a devastating disease of potato. Our previous work illustrated that scopolamine, the main bioactive substance of extract, exerts direct inhibitory effects on , but it is unclear whether scopolamine and extract can boost resistance to late blight in potato. In this study, .
View Article and Find Full Text PDFBambara Bean Substitution Improves the Nutritional Content and Increases the Satiety Index of Purple Sweet Potato Bread.
Prev Nutr Food Sci
December 2024
Department of Community Nutrition, Faculty of Human Ecology, IPB University, Bogor 16680, Indonesia.
This study aimed to develop tuber bread from purple sweet potato and bambara beans with high satiety and low glycemic index (GI). Different ratios of purple sweet potato to bambara bean were used: 100:0 (F0), 80:20 (F1), 60:40 (F2), and 40:60 (F3). The satiety index (SI) was determined by assessing the consumption of a 240 kcal isocaloric food and collecting data through a visual analog scale.
View Article and Find Full Text PDFEfficient detection of eyes on potato tubers using deep-learning for robotic high-throughput sampling.
Front Plant Sci
December 2024
Center for Precision and Automated Agricultural Systems, Department of Biological Systems Engineering, Washington State University, Prosser, WA, United States.
Molecular-based detection of pathogens from potato tubers hold promise, but the initial sample extraction process is labor-intensive. Developing a robotic tuber sampling system, equipped with a fast and precise machine vision technique to identify optimal sampling locations on a potato tuber, offers a viable solution. However, detecting sampling locations such as eyes and stolon scar is challenging due to variability in their appearance, size, and shape, along with soil adhering to the tubers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!