AI Article Synopsis
- Researchers transfected embryonic stem cells with alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA controlled by the beta-actin, cytomegalovirus enhancer/promoter.
- Positive clones with integrated cDNA showed higher efficiency in differentiating into myocardial cells compared to negative clones or parental cells.
- Myocardial cells from positive clones exhibited greater survival rates, suggesting that the Lewis X structure produced by Fuc T IV plays a key role in enhancing myocardial differentiation, with integrin action potentially involved in the mechanism.
Article Abstract
alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (negative clones) or parental cells. Furthermore, myocardial cells differentiated from the positive clones survived longer than those differentiated from the negative clones or parental cells. These results indicate that Lewis X structure, the product of Fuc T IV, enhances myocardial differentiation. The mechanism of the phenomenon is discussed on relation to integrin action.
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| http://dx.doi.org/10.1247/csf.22.247 | DOI Listing |
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