We describe here an efficient procedure for the precise quantitation of leukotriene E4 (LTE4) in a small volume of urine, which was achieved mainly by the use of an Empore extraction disk cartridge. After addition of [3H]LTE4 to 2 ml of urine, an Empore C18 cartridge was used for initial extraction of the urine, which resulted in the extraction of LTE4 in a small volume of solvent. The eluate could then be injected onto a high-performance liquid chromatography column without further concentration. After separation by high-performance liquid chromatography, LTE4 was extracted from the effluent using an Empore C18 cartridge. The concentration of LTE4 was subsequently quantified by enzyme immunoassay. LTE4 can be recovered from urine with sufficient efficiency (69.9+/-4.7%, mean+/-S.D., n=101). The coefficient of variation of the assay procedure was less than 10%. When urine was spiked with different amounts of LTE4, the recovery of LTE4 added to the urine specimen was less than 120%. The concentration of LTE4 in urine from normal healthy subjects was 48.0+/-15.3 pg/mg creatinine (n=15).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0378-4347(97)00006-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Optimization and evaluation of protein C-terminal peptide enrichment strategy based on arginine cleavage].
Se Pu
January 2022
School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.
As unique biomarkers, protein C-termini are involved in various biological processes such as protein trafficking, subcellular relocation, and signal transduction. Dysregulation of protein C-terminal status is critical during the development of various diseases, including cardiovascular, neurodegenerative, and metabolic diseases and cancer. Thus, global profiling of protein C-termini is of great value in providing mechanistic insight into biological or pathological processes, as well as for identifying potential new targets for therapeutic treatment.
View Article and Find Full Text PDFApplication of Passive Sampling to Characterise the Fish Exometabolome.
Metabolites
February 2017
Centre for Environment, Fisheries and Aquaculture Science, Cefas Weymouth Laboratory, Weymouth, Dorset DT4 8UB, UK.
The endogenous metabolites excreted by organisms into their surrounding environment, termed the exometabolome, are important for many processes including chemical communication. In fish biology, such metabolites are also known to be informative markers of physiological status. While metabolomics is increasingly used to investigate the endogenous biochemistry of organisms, no non-targeted studies of the metabolic complexity of fish exometabolomes have been reported to date.
View Article and Find Full Text PDFDetermination of selected nitropolycyclic aromatic hydrocarbons in water samples.
Chem Pharm Bull (Tokyo)
July 2014
Graduate School of Medical Sciences, Division of Pharmaceutical Sciences, Kanazawa University.
A new method for the analysis of selected nitropolycyclic aromatic hydrocarbons (NPAHs) at ultra-trace levels in water samples is proposed. Particulate NPAHs were collected on a GC glass fiber filter. Soluble NPAHs were collected on a C18 Empore disk.
View Article and Find Full Text PDFDetermination of chlorobenzenes in water samples by solid-phase disk extraction and gas chromatography-electron capture detection.
J Chromatogr Sci
May 2015
1Zhejiang Province Key Lab of Mariculture & Enhancement, Marine Fishery Institute of Zhejiang Province, Zhoushan 316100, China.
A simple, rapid, sensitive and high throughput method is described, based on solid-phase disk extraction (SPDE) and gas chromatography-electron capture detection, for the determination of chlorobenzens (CBs) in water samples. The proposed SPDE sample pretreatment method was initially optimized and the optimum experimental conditions were found to be as follows: 500 mL water sample (pH 2.5) extracted and enriched by an Empore 3-stn C18 (octadecyl) SPE disk at flow rate of 5 to 50 mL/min, eluted by 5 mL of acetone and 3 × 5 mL of methylene dichloride.
View Article and Find Full Text PDFDetection of Δ9-tetrahydrocannabinol in exhaled breath collected from cannabis users.
J Anal Toxicol
October 2011
Department of Medicine, Section of Clinical Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Exhaled breath has recently been proposed as a new possible matrix for drugs of abuse testing. A key drug is cannabis, and the present study was aimed at investigating the possibility of detecting tetrahydrocannabinol and tetrahydrocannabinol carboxylic acid in exhaled breath after cannabis smoking. Exhaled breath was sampled from 10 regular cannabis users and 8 controls by directing the exhaled breath by suction through an Empore C(18) disk.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!