Enterovirus genomes in wastewater: concentration on glass wool and glass powder and detection by RT-PCR.

J Virol Methods

Laboratoire de Virologie, Faculté de Pharmacie, Nancy, France.

Published: May 1997

AI Article Synopsis

  • Standard methods for detecting enteroviruses in water are slow and costly because they rely on cell culture.
  • The study shows that using glass powder or glass wool to concentrate viruses is effective for detecting viral genomes in treated wastewater using RT semi-nested PCR.
  • While no enterovirus genomes were found directly in treated wastewater samples, significant percentages were detected after concentration, with glass powder yielding 72% positivity after additional flocculation.

Article Abstract

Standard methods for detecting enteroviruses in environmental samples require cell culture, which is time consuming and expensive. The reverse transcription-polymerase chain reaction (RT-PCR) is a rapid, sensitive method for detecting enteroviruses in water. However, environmental samples often contain substances that inhibit PCR amplification of target RNA. Hence the virus must be concentrated by procedures that do not interfere with amplification. This study shows that virus concentration by adsorption onto glass powder or glass wool supports is suitable for detecting viral genomes in treated wastewater by RT semi-nested PCR. No enterovirus genome was detected directly in 25 samples of treated wastewater by RT semi-nested PCR. However, samples concentrated by adsorption onto glass wool or glass powder showed that 48% (glass powder) and 56% (glass wool) contained virus. Secondary concentration by organic flocculation was unsuitable for detecting virus concentrated on glass wool (20% positive samples), but it helped to increase the detection of the genome after concentration on glass powder (72% positive samples).

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http://dx.doi.org/10.1016/s0166-0934(97)02193-9DOI Listing

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