AI Article Synopsis
- A new type of immunosensor has been developed using biocomposite materials made from graphite powder, rabbit IgG, and resins, which serve both as a reservoir for biological molecules and a transducer.
- To evaluate its analytical capabilities, a competitive binding assay was created to measure the presence of rabbit IgG in a sample, utilizing goat anti-rabbit IgG labeled with an enzyme for detection.
- The immunosensor's surface can be easily regenerated through polishing, allowing for repeated use in subsequent assays.
Article Abstract
Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.
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| http://dx.doi.org/10.1021/ac961222b | DOI Listing |
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