We identified two new genes (scarf and collar) of planarians using subtracting hybridization. mR-NAs of these genes are distributed in different ways in regeneration blastemas of different polarity. Zone-specific expression of these genes in non-regenerating planarians has been demonstrated. The level of expression of the identified genes in the head regeneration buds of the first-third days of regeneration was lower than that in the corresponding intact tissues and tail regeneration buds.
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