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Spatio-temporal dynamics and risk cluster analysis of highly pathogenic avian influenza (H5N1) in poultry: Advancing outbreak management through customized regional strategies in Egypt.
Open Vet J
November 2024
Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt.
Background: Highly pathogenic avian influenza (HPAI) (H5N1) has been endemic in Egypt for almost two decades, profoundly impacting both the poultry industry and public health. Egypt stands as a prominent epicenter for HPAI H5N1 outbreaks in Africa, marked by the highest number of positive human cases. Despite continuous governmental efforts, prior research underscored the inadequacy of strategies in controlling the virus spread.
View Article and Find Full Text PDFEvaluation of a 70 kDa Excreted/Secreted Coproantigen Immunoassay for the Detection of in Dogs.
Iran J Parasitol
January 2024
Department of Invertebrates Zoology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México.
Background: We aimed to develop a sandwich ELISA, using polyclonal antibodies against excretory/secretory (E/S) antigens specific to coproantigens present in -positive dogs.
Methods: Antibodies were produced at Biological Sciences School, Autonomous University of Nuevo León, in 2023 by immunization of rabbits with antigenic extracts from in vitro cultures of larvae. Assays were performed on 100 stool samples from pet dogs, measuring sensitivity, specificity, and cross-reactivity against other parasitic infections.
Expression and purification of recombinant tilapia lake virus segment 4 protein and its in-vitro biological activity for potential use in vaccine development.
Sci Rep
December 2024
Department of Biotechnology, Faculty of Agro-industry, Kasetsart University, Bangkok, 10900, Thailand.
Tilapia lake virus (TiLV) disease is highly contagious and causes substantial mortality in tilapia. Currently, no effective treatments or commercial vaccines are available to prevent TiLV infection. In this study, TiLV segment 4 (S4) was cloned into the pET28a(+)vector and transformed into Escherichia coli BL21(DE3).
View Article and Find Full Text PDFSingle-chain antibody gene therapy strategy based on high-throughput screening triggers sustained antiviral activity in the body.
J Virol
December 2024
College of Animal Science and Technology, Northwest A&F University College of Animal Science and Technology, Yangling, China.
The occurrence of viral diseases poses a huge threat and impact on human public health safety and the development of the animal and fishery industry. Here, a strain of single-chain antibody fragment, scFv-1, was isolated from the phage antibody display library construct by immunizing New Zealand white rabbits with rhabdovirus. analysis showed that the single-chain antibody could inhibit the infection of the virus in multiple pathways, including adsorption, fusion, and release.
View Article and Find Full Text PDFIntra-individual polymorphisms in the mitochondrial COI gene of tick-killing Ixodiphagus wasps parasitizing Haemaphysalis flava ticks.
Acta Trop
December 2024
Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan; Division of Parasitology, Veterinary Research Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan. Electronic address:
Ixodid ticks are significant vectors of pathogens affecting both humans and animals. Biological control with natural enemies represents a sustainable tool for managing ticks. However, there is a substantial lack of knowledge about the natural enemies of ticks.
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