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Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-FeO/FeO nanosheets for APOE ε4 genes ultrasensitive detection.
Bioelectrochemistry
February 2025
School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China. Electronic address:
A label-free electrochemical DNA detection strategy based on self-assembled α-FeO/FeO nanosheets with PNA-DNA hybridization process was developed for ultrasensitive detection of APOE ε4 gene, one of the most robust genetic risks for Alzheimer's Disease (AD). In this work, magnetic α-FeO/FeO heterogeneous nanosheets were prepared by hydrothermal-calcined reduction method and loaded with Au nanoparticles (AuNPs) on their surfaces. The magnetic α-FeO/FeO@Au nanocomposites significantly enhanced the electrochemical response as a signal amplification matrix and were able to bind to the magnetic glassy carbon electrode (MGCE) surface by magnetic self-assembly.
View Article and Find Full Text PDFIntegration of silicon nanostructures for health and energy applications using MACE: a cost-effective process.
Nanotechnology
August 2024
FlexMEMS Research Centre (FMRC), Manipal University Jaipur, Jaipur, Rajasthan 303007, India.
Silicon in its nanoscale range offers a versatile scope in biomedical, photovoltaic, and solar cell applications. Due to its compatibility in integration with complex molecules owing to changes in charge density of as-fabricated Silicon Nanostructures (SiNSs) to realize label-free and real-time detection of certain biological and chemical species with certain biomolecules, it can be exploited as an indicator for ultra-sensitive and cost-effective biosensing applications in disease diagnosis. The morphological changes of SiNSs modified receptors (PNA, DNA, etc) have huge future scope in optimized sensitivity (due to conductance variations of SiNSs) of target biomolecules in health care applications.
View Article and Find Full Text PDFFuran-modified PNA probes for covalent targeting and ligation of nucleic acids.
Methods
October 2023
Organic and Biomimetic Chemistry Research Group, Ghent University, Krijgslaan 281 S4, B-9000 Ghent, Belgium. Electronic address:
While natural oligonucleotides (ONs) are increasingly used as therapeutic and diagnostic tools, they still face certain challenges such as low resistance to enzymatic degradation, potential immunogenicity, and delivery issues, which can limit their applications. Peptide Nucleic Acids (PNAs) are promising alternatives due to their high affinity for DNA and RNA, the high resistance to enzymatic degradation, and the easy introduction of a wide range of potential modifications. Chemical modifications that enable the covalent targeting of specific DNA and RNA strands offer additional advantages, including enhanced potency.
View Article and Find Full Text PDFA highly sensitive and robust electrochemical biosensor for microRNA detection based on PNA-DNA hetero-three-way junction formation and target-recycling catalytic hairpin assembly amplification.
Talanta
January 2024
School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China. Electronic address:
Robust and sensitive methods for the detection of microRNAs (miRNAs) are crucial in the clinical diagnosis of cancers. In this study, a novel electrochemical biosensor with high sensitivity for miRNA-21 detection is developed, which relies on the formation of a peptide nucleic acid (PNA)-DNA hetero-three-way junction (H3WJ) and target-recycling catalytic hairpin assembly (CHA) amplification. The electroneutral PNA probes are initially immobilized onto a gold electrode to construct the sensor.
View Article and Find Full Text PDFLocalization of Mutations in Non-small-cell Lung Cancer Tissues Using Mutation-specific PNA-DNA Probes.
Cancer Genomics Proteomics
October 2023
Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
Background/aim: Epidermal growth factor receptor (EGFR) signaling inhibitors are potent therapeutic agents for EGFR-mutant non-small-cell lung cancer, but the effects of such inhibitors on the localization of EGFR mutations in tumor tissues remain to be elucidated. Thus, a simple and efficient technology for the detection of mutations in tumor tissue specimens needs to be developed.
Materials And Methods: Using an EGFR mutation-specific peptide nucleic acid (PNA)-DNA probe, the EGFR mutation-positive part of whole NSCLC tissues was visualized by immunofluorescence.
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