An Aspergillus terreus gene (arg1) encoding ornithine carbamoyltransferase (OCTase) has been cloned and sequenced. The deduced amino acid sequence contains a signal peptide suggestive of a mitochondrial location for the A. terreus enzyme. Alignment of the A. terreus OCTase sequence with other OCTases revealed the presence of conserved regions. Northern analysis indicates that arg1 expression is regulated at the level of transcription and that transcription of the arg1 gene is not markedly affected by arginine sufficiency. However, histidine starvation effected by 3-amino-1,2,4-triazole increased transcription of the arg1 gene, indicating cross-pathway regulation of OCTase synthesis in A. terreus.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1574-6968.1997.tb10330.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Arginase 1 genetic variation is associated with the risk of vascular complications in type 2 diabetes.
Biomark Med
January 2025
Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai, China.
Objective: This study aims to explore the association between arginase 1 (ARG1) genetic variation and susceptibility to type 2 diabetes (T2DM) vascular complications, a primary cause of morbidity and mortality in diabetics.
Methods: ARG1, a risk gene for cardiovascular disease, was identified from GEO datasets GSE22255 and GSE58294. The ENCODE database identified four candidate single-nucleotide polymorphism (SNP) loci.
Arginase-1-specific T cells target and modulate tumor-associated macrophages.
J Immunother Cancer
January 2025
National Center for Cancer Immune Therapy (CCIT-DK), Department of Oncology, Herlev Hospital, Herlev, Denmark
Background: Arginase-1 (Arg1) expressing tumor-associated macrophages (TAMs) may create an immune-suppressive tumor microenvironment (TME), which is a significant challenge for cancer immunotherapy. We previously reported the existence of Arg1-specific memory T cells among peripheral blood mononuclear cells (PBMCs) and described that Arg-1-based immune modulatory vaccines (IMVs) control tumor growth and alter the M1/M2 macrophage ratio in murine models of cancer. In the present study, we investigated how Arg1-specific T cells can directly target TAMs and influence their polarization.
View Article and Find Full Text PDFSex-specific transcriptomic profiling reveals key players in bone loss associated with Alzheimer's disease.
Geroscience
January 2025
Division of Endocrinology, Department of Medicine, Augusta University, Augusta, GA, USA.
Alzheimer's disease (AD), a progressive neurodegenerative disorder, is frequently associated with musculoskeletal complications, including sarcopenia and osteoporosis, which substantially impair patient quality of life. Despite these clinical observations, the molecular mechanisms linking AD to bone loss remain insufficiently explored. In this study, we examined the femoral bone microarchitecture and transcriptomic profiles of APP/PS1 transgenic mouse models of AD to elucidate the disease's impact on bone pathology and identify potential gene candidates associated with bone deterioration.
View Article and Find Full Text PDFThe impact of cytokines and tumour-conditioned medium on the properties of murine in vitro generated myeloid-derived suppressor cells.
Scand J Immunol
February 2025
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of immature myeloid cells playing a critical role in immune suppression. In vitro-generated MDSCs are a convenient tool to study the properties of tumour-associated MDSCs. Here, we compared six protocols for in vitro generation of functional mouse MDSCs from bone marrow progenitors.
View Article and Find Full Text PDFSynthesis of Arginase Inhibitors: An Overview.
Pharmaceutics
January 2025
Department of Pharmacy, "Federico II" University of Naples, 80131 Naples, Italy.
Arginase (ARG) is a binuclear manganese-containing metalloenzyme that can convert L-arginine to L-ornithine and urea and plays a key role in the urea cycle. It also mediates different cellular functions and processes such as proliferation, senescence, apoptosis, autophagy, and inflammatory responses in various cell types. In mammals, there are two isoenzymes, ARG-1 and ARG-2; they are functionally similar, but their coding genes, tissue distribution, subcellular localization, and molecular regulation are distinct.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!