The spread of HIV infection into leishmaniasis endemic areas has increased the incidence of immunosupressed patients with kalaazar in Portugal. The dermotropic zymodeme MON-24 of leishmania infantum has been already isolated from a Portuguese AIDS patient, as in some other Mediterranean countries. In this paper we report the isolation of L. donovani MON-18 from a drug addicted Portuguese patient with clinical visceral leishmaniasis and AIDS, that suggests a mechanically transmitted infection by the use of a shared needle or syringe.
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http://dx.doi.org/10.1051/parasite/1994014391 | DOI Listing |
Microbes Infect
March 2007
Institute of Microbiology and Hygiene (Parasitology), Charité Universitätsmedizin Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
Multilocus enzyme electrophoresis (MLEE) is the gold standard for taxonomy and strain typing of Leishmania, but has some limitations. An alternative reliable and fast genotyping method for addressing population genetic and key epidemiological questions, is multilocus microsatellite typing (MLMT). MLMT using 15 markers was applied to 91 strains of L.
View Article and Find Full Text PDFTrans R Soc Trop Med Hyg
April 2001
Department of Epidemiology and Clinical Sciences, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
From the early 1900s, visceral leishmaniasis (VL; kala-azar) has been among the most important health problems in Sudan, particularly in the main endemic area in the eastern and central regions. Several major epidemics have occurred, the most recent--in Western Upper Nile province in southern Sudan, detected in 1988--claiming over 100,000 lives. The disease spread to other areas that were previously not known to be endemic for VL.
View Article and Find Full Text PDFTrans R Soc Trop Med Hyg
August 1998
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L.
View Article and Find Full Text PDFFolia Parasitol (Praha)
August 1996
Instituto de Higiene e Medicina Tropical, Centro de Malária e Outras Doenças Tropicais, Lisboa, Portugal.
The mechanisms which permit Leishmania to survive inside macrophages are not totally understood although it is known that prolonged culture in vitro results in loss of virulence. One of the cell surface molecules often implicated in virulence mechanisms is the glycoprotein of 63 kDa (gp63). In this work we studied changes in infectivity of L.
View Article and Find Full Text PDFParasite
December 1994
Instituto de Higiene e Medicina Tropical (IHMT), Lisboa, Portugal.
The spread of HIV infection into leishmaniasis endemic areas has increased the incidence of immunosupressed patients with kalaazar in Portugal. The dermotropic zymodeme MON-24 of leishmania infantum has been already isolated from a Portuguese AIDS patient, as in some other Mediterranean countries. In this paper we report the isolation of L.
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