Some metabolic peculiarities of bacteria of the genus Klebsiella were investigated. The bacteria under study were isolated from different sources and varied in virulence. The pathogenic and saprotrophic enterobacteria were discriminated based on their response to the addition of carbohydrates or nitrate to the medium. Pathogenic Klebsiella spp. exhibited mainly the mixed formic-acid type of fermentation and were more resistant to nitrates than saprotrophic bacteria with the butanediol type of fermentation. The bacteria Klebsiella pneumoniae subsp. pneumoniae isolated from different sources, such as patients, healthy persons, or the environment, exhibited no substantial differences in metabolism and virulence. It was inferred that these bacteria themselves cannot cause disease, and their isolation in morbid states is an indirect result of dysbacteriosis.
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Nat Methods
January 2025
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
A key challenge of the modern genomics era is developing empirical data-driven representations of gene function. Here we present the first unbiased morphology-based genome-wide perturbation atlas in human cells, containing three genome-wide genotype-phenotype maps comprising CRISPR-Cas9-based knockouts of >20,000 genes in >30 million cells. Our optical pooled cell profiling platform (PERISCOPE) combines a destainable high-dimensional phenotyping panel (based on Cell Painting) with optical sequencing of molecular barcodes and a scalable open-source analysis pipeline to facilitate massively parallel screening of pooled perturbation libraries.
View Article and Find Full Text PDFJ Infect
January 2025
National Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong Agricultural University, Wuhan, PR China; Hubei Hongshan Laboratory, Wuhan, PR China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, PR China. Electronic address:
Objectives: Emerging human pathogens of animal origin have become an increasing public health concern in recent years. The aim of this study was to investigate the transmission of group B streptococcus (GBS) clonal complex (CC) 61 strains in the southern Chinese population and analyze their genetic characteristics.
Methods: Whole-genome sequencing was performed on 693 clinical isolates of GBS collected from southern China between 2016 and 2021, and the prevalence of human CC61 isolates was investigated by genomic epidemiology.
Plant Dis
January 2025
50 Yonsei-ro, Seodaemun-guSeoul, Korea (the Republic of), 03722;
Fire blight, a devastating bacterial disease affecting rosaceous plants such as apples and pears, is caused by . The disease, known for its rapid spread and destructive potential, can lead to severe symptoms and often result in the death of infected plants. In Korea, the observation of was first recorded in 2015, and subsequent dissemination has been noted across the peninsula.
View Article and Find Full Text PDFInt J Radiat Biol
January 2025
Department of Horticulture, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
Purpose: The study focused on developing a rapid PCR-based detection method and employing gamma irradiation techniques to manage , aiming to produce brown rot-free export-quality potatoes. This initiative seeks to enhance potato exports from Bangladesh.
Materials And Methods: Samples of potato tubers and soil were collected from various commercially significant potato-growing areas, resulting in a total of 168 isolates from potato tubers and soil across 12 regions.
Microbiol Spectr
January 2025
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Unlabelled: Bovine herpesvirus (BoHV) infection poses a significant threat to the healthy development of the cattle industry. BoHV-1 primarily causes infectious bovine rhinotracheitis, while BoHV-5 is associated with bovine necrotic meningoencephalitis. These two pathogens not only exhibit a high correlation in antigenicity and genetic background but, more importantly, can establish latent infections within the bovine ganglion.
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