AI Article Synopsis
- The study aimed to determine if free gp120 can be found on the surface of apoptotic CD4+ T lymphocytes in lymph nodes of HIV-positive patients.
- The methodology involved analyzing lymph-node cell samples from three HIV-positive individuals using immunogold-labeling techniques to identify cell types and the presence of gp120.
- Results showed that free gp120 was more frequently associated with apoptotic CD4+ T cells than with normal ones and was not found on CD8+ T cells or B cells, suggesting that gp120 may contribute to the premature apoptosis of CD4+ T lymphocytes in individuals with HIV.
Article Abstract
Objective: To study whether free gp120 can be detected on the plasma membranes of apoptotic CD4+ T lymphocytes in lymph nodes from HIV-positive patients.
Methods: Lymph-node cell suspensions prepared from three HIV-positive patients were studied by pre-embedding, double-immunogold-labeling to identify cell type, determine cell morphology, and detect the presence of bound gp120 molecules. Cells were classified by their surface antigens as helper/inducer T lymphocytes (CD4+), cytotoxic/suppressor T lymphocytes (CD8+), B cells (CD20+), and total lymphocytes [CD45+, leukocyte common antigen (LCA)+].
Results: gp120 colabelled with both apoptotic and normal CD4+ T lymphocytes and LCA+ cells, but not with either apoptotic or normal CD8+ T lymphocytes or B cells. gp120 was more often identified on apoptotic than on normal CD4+ T lymphocytes. The gp120 and CD45 label were often colocalized. HIV particles were not identified to be associated with or budding from either normal or apoptotic lymphocytes.
Conclusions: Free gp120 is found bound to CD4+ T cells in lymph nodes of HIV-infected individuals and potentially mark them for premature death by apoptosis.
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| http://dx.doi.org/10.1097/00002030-199701000-00005 | DOI Listing |
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