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The mode of action of a purified toxin from Naja naja naja (Indian cobra) venom was investigated in frog rectus abdominis muscle, chick biventer cervicis muscle, cat tibialis anterior muscle (close-arterial) and in both innervated and denervated rat diaphragm muscle preparations. The toxin inhibited the acetylcholine responses of rectus abdominis muscle. The inhibition was antagonized by neostigmine and increasing concentrations of acetylcholine, suggesting a competitive binding of the toxin to cholinergic receptors.
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