AI Article Synopsis
- Formation of araB-lacZ fusions is a complex adaptive mutation process, with 84 sequenced fusions showing standard MuR linker sequences from the Mu DNA rearrangement process.
- Different isolation methods resulted in standard and non-standard fusions, suggesting that cellular conditions can impact the fusion outcome.
- Each fusion exhibited unique DNA junctions, supporting the idea that the formation of these fusions is a multi-dimensional cell biology process rather than a single biochemical event.
Article Abstract
Formation of araB-lacZ coding-sequence fusions is a key adaptive mutation system. Eighty-four independent araB-lacZ fusions were sequenced. All fusions carried rearranged MuR linker sequences between the araB and lacZ domains indicating that they arose from the standard intermediate of the well-characterized Mu DNA rearrangement process, the strand transfer complex (STC). Five non-standard araB-lacZ fusions isolated after indirect sib selection had novel structures containing back-to-back inverted MuR linkers. The observation that different isolation procedures gave rise to standard and non-standard fusions indicates that cellular physiology can influence late steps in the multi-step biochemical sequence leading to araB-lacZ fusions. Each araB-lacZ fusion contained two novel of DNA junctions. The MuR-lacZ junctions showed 'hot-spotting' according to established rules for Mu target selection. The araB-MuR and MuR-MuR junctions all involved exchanges at regions of short sequence homology. More extensive homology between MuR and araB sequences indicates potential STC isomerization a resolvable four-way structure analogous to a Holliday junction. These results highlight the molecular complexity of araB-lacZ fusion formation, which may be thought of as a multi-step cell biology process rather than a unitary biochemical reaction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1365-2958.1997.3031666.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual fluorescence system for flow cytometric analysis of Escherichia coli transcriptional response in multi-species context.
J Microbiol Methods
February 2009
School of Biological Sciences, Nanyang Technological University, Singapore.
When studying interspecies interactions in a bacterial consortium, it may be desirable to analyze one species' transcriptional response as influenced by the other species. We developed a dual fluorescence system of Escherichia coli for Fluorescence-Activated Cell Sorter (FACS)-based analysis for such a purpose. First, we generated E.
View Article and Find Full Text PDFStarvation-induced Mucts62-mediated coding sequence fusion: a role for ClpXP, Lon, RpoS and Crp.
Mol Microbiol
April 1999
Laboratoire de Génétique des Procaryotes, Département de Biologie Moléculaire, Université Libre de Bruxelles, 67 rue des Chevaux, B1640 Rhode St Genèse, Belgium.
The formation of araB-lacZ coding sequence fusions in Escherichia coli is a particular type of chromosomal rearrangement induced by Mucts62, a thermoinducible mutant of mutator phage Mu. Fusion formation is controlled by the host physiology. It only occurs after aerobic carbon starvation and requires the phage-encoded transposase pA, suggesting that these growth conditions trigger induction of the Mucts62 prophage.
View Article and Find Full Text PDFH-NS and RpoS regulate emergence of Lac Ara+ mutants of Escherichia coli MCS2.
J Bacteriol
July 1997
Servicio de Microbiologia, Hospital Ramón y Cajal, INSALUD, Madrid, Spain.
Two master growth-phase regulatory proteins, H-NS and sigmaS, are involved in the formation of araB-lacZ fusion clones of Escherichia coli MCS2. The stationary-phase sigma factor RpoS is strictly required for the appearance of such mutants, whereas the histone-like protein H-NS represses their emergence. Our results support the idea that genetic changes leading to adaptive mutation in this model system are regulated by physiological signal transduction networks.
View Article and Find Full Text PDFDifferent structures of selected and unselected araB-lacZ fusions.
Mol Microbiol
March 1997
Université Libre de Bruxelles, Department of Molecular Biology, Rhode-St-Genése, Belgium.
Article Synopsis
- Formation of araB-lacZ fusions is a complex adaptive mutation process, with 84 sequenced fusions showing standard MuR linker sequences from the Mu DNA rearrangement process.
- Different isolation methods resulted in standard and non-standard fusions, suggesting that cellular conditions can impact the fusion outcome.
- Each fusion exhibited unique DNA junctions, supporting the idea that the formation of these fusions is a multi-dimensional cell biology process rather than a single biochemical event.
Genome organization, natural genetic engineering and adaptive mutation.
Trends Genet
March 1997
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA.
Bacterial evolution is considered in the light of molecular discoveries about genome organization, biochemical mechanisms of genetic change, and cellular control networks. Prokaryotic genetic determinants are organized as modular composites of coding sequences and protein-factor binding sites joined together during evolution. Studies of genetic change have revealed the existence of biochemical functions capable of restructuring the bacterial genome at various levels and joining together different sequence elements.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!