Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
In this work the results of the genotyping of HIV strains obtained from 12 patients residing in Russia are presented. Proviral DNA from lysates of mononuclear cells was amplified with the use of primers, complementary to the conservative site of gene gag p7/p9 with the subsequent sequencing of the amplified material and phylogenetic analysis. 9 strains were classified with subtype B (most frequently occurring in Western countries), 2 of them being found closely related (probably, of African origin). In 2 patients infected from the same source the virus of unusual subtype F/1 was identified. This is the second case of the detection of this subtype in the world. One strain belonged to subtype G. in spite of the fact that in accordance with the epidemiological history only 3 patients got infection outside Russia, molecular epidemiological studies showed the genetic heterogeneity of the circulating strains and suggested the diversity of sources of the penetration of HIV infection to Russia.
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