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Human recombinant antibody fragments specific for a rye-grass pollen allergen: characterization and potential applications.
Mol Immunol
September 1996
Department of Biological and Technological Research, San Raffaele Scientific Institute, Milano, Italy.
One of the major allergens from the pollen of perennial rye grass (Lolium perenne), Lol pII, was used to isolate specific antibody fragments from a random combinatorial library displaying a large repertoire of human Fab on filamentous phages. After five panning cycles on recombinant Lol pII immunotubes, phage binders were isolated and the antibody fragments expressed as soluble Fab molecules in the Escherichia coli periplasm. The DNA sequencing of the clones producing antibodies with the highest binding activity showed three of them to be identical, while one differed by two amino acid substitutions in the heavy chain.
View Article and Find Full Text PDFLol pII allergen. Production and characterization of the recombinant protein and human antibody fragments.
Adv Exp Med Biol
July 1997
Dibit, Department of Biology and Technology, San Raffaele Scientific Institute, Milano, Italy.
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