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The biochemical characters of eight strains identified as Citrobacter sedlakii were investigated with the aid of six methods (tube tests, API 50 CH, API 20 E, MICROSCAN, BIOLOG, and CRYSTAL). All the strains were well defined on the basis of biochemical properties investigated with the aid of laboratory-prepared tube tests. The results obtained by the identification kits could not be correctly interpreted. Commercial diagnostic kits should include the reference data necessary for the identification of new Citrobacter species.
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