The effects of infection by the human cytomegaloviruses Ad-169 on the incorporation of [14C]acetate into the polar and neutral lipids of human embryonic lung cells and human saphenous vein smooth muscle cells were compared to [14C]acetate incorporation in mock-infected control cells. Cytomegalovirus infection caused a shift in the relative amounts of polar and neutral lipids, with infected cells having lower amounts of polar lipids and higher amounts of neutral lipids than mock-infected controls. When neutral lipids were separated into diglyceride (DG), cholesterol (C), fatty acid, triglyceride (TG) and cholesterol ester (CE) components, Ad-169-infected cells had lower levels of incorporation of label into CE, TG, and DG fractions, and higher levels of label incorporation into C than mock-infected cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000150521 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the putative yeast mitochondrial triacylglycerol lipase Tgl2.
J Biol Chem
January 2025
Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany. Electronic address:
Mitochondria derive the majority of their lipids from other organelles through contact sites. These lipids, primarily phosphoglycerolipids, are the main components of mitochondrial membranes. In the cell, neutral lipids like triacylglycerides (TAGs) are stored in lipid droplets, playing an important role in maintaining cellular health.
View Article and Find Full Text PDFSelection of In Vivo Relevant Dissolution Test Parameters for the Development of Cannabidiol Formulations with Enhanced Oral Bioavailability.
Pharmaceutics
January 2025
Laboratory of Pharmaceutical Technology and Biopharmacy, Center for Interdisciplinary Research on Medicines (CIRM), University of Liège, 4000 Liège, Belgium.
Cannabidiol (CBD) shows interesting therapeutic properties but has yet to demonstrate its full potential in clinical trials partly due to its low solubility in physiologic media. Two different formulations of CBD (amorphous and lipid-based) have been optimized and enable an increase in bioavailability in piglets. In vivo studies are time-consuming, costly and life-threatening.
View Article and Find Full Text PDFChemical Characterization and Differential Lipid-Modulating Effects of Selected Plant Extracts from Côa Valley (Portugal) in a Cell Model for Liver Steatosis.
Pharmaceuticals (Basel)
January 2025
Clinic Academic Center of Coimbra (CACC), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal.
Background/objectives: Côa Valley, located in the northeast of Portugal, harbors more than 500 medicinal plant species. Among them, four species stand out due to their traditional uses: Desf. (hemorrhages, urethritis, hepatitis), L.
View Article and Find Full Text PDFType I Interferon (IFNd) enhanced the innate immune response and lipid droplets (LDs) formation in obscure puffer Takifugu obscurus.
Dev Comp Immunol
January 2025
Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, Hohai University, Nanjing 210024, China; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210024, China. Electronic address:
As one member of type I IFN, IFNd showed huge distinctive responses and activity during the viral or bacterial infection in various fish species. Our previous study identified IFNd from obscure puffer Takifugu obscurus (ToIFNd), and the function and regulation of ToIFNd was further investigated in the present study. The transcriptional levels of ToIFNd were significantly induced post bacteria Vibrio harveyi and virus simulator Poly (I:C) stimulation in the head kidney.
View Article and Find Full Text PDFNeutral lipids restrict the mobility of broken DNA molecules during comet assays.
Biol Cell
January 2025
Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, Centre National de la Recherche Scientifique, Montpellier, France.
One widespread technique to assess in relative terms the amount of broken DNA present in the genome of individual cells consists of immobilizing the cell's nucleus under an agarose pad (called the nucleoid) and subjecting the whole genome to electrophoresis to force broken DNA molecules out of it. Since the migrating broken DNA molecules create a tail behind the nucleoid, this technique is named the comet assay. While performing comet assays regularly, we systematically observed circular regions devoid of DNA within the nucleoid region.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!