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Recent advances in biocatalytic and chemoenzymatic synthesis of oligonucleotides.
Chembiochem
January 2025
Institut Pasteur, Department of Structural Biology and Chemistry, 28 Rue du Dr. Roux, 75015, Paris, FRANCE.
Access to synthetic oligonucleotides is crucial for applications in diagnostics, therapeutics, synthetic biology, and nanotechnology. Traditional solid phase synthesis is limited by sequence length and complexities, low yields, high costs and poor sustainability. Similarly, polymerase-based approaches such as in vitro transcription and primer extension reactions do not permit any control on the positioning of modifications and display poor substrate tolerance.
View Article and Find Full Text PDFReimagining André Rey's method for recording the copy process of the Rey Complex Figure Test: A commentary.
J Neuropsychol
January 2025
Department of Aged Care and Rehabilitation, Liverpool Hospital, Sydney, New South Wales, Australia.
In 1941, André Rey published the Rey Complex Figure, a widely used test for assessing visual-constructional ability and visual memory. It consists of two parts: copy and recall. Evaluating the copy portion presents challenges, as it requires the administrator to focus on both the process and outcome.
View Article and Find Full Text PDFAlternative and Sustainable Route to Explore a New Class of Amidines by Photochemical Synergistic Effect of Copper/Nitroxyl Radical Catalysis via Halogen-Atom Transfer.
Chemistry
January 2025
VIT University, Materials Chemistry Division, School of Advanced Sciences, VIT University, 632014, Vellore, INDIA.
Amidines are a vital class of bioactive compounds and often necessitate multiple components for their synthesis. Therefore, exploring efficient and sustainable methodologies for their synthesis is indispensable. Herein, we disclose an alternative and greener method for synthesizing an unexplored new class of amidines through the photochemical synergistic effect of copper/nitroxyl radical catalysis.
View Article and Find Full Text PDFThoracic Electric Impedance Tomography Detects Lung Volume Changes in Amyotrophic Lateral Sclerosis.
Muscle Nerve
January 2025
Division of Neurology, Department of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Introduction/aims: Spirometry is the conventional means to measure lung function in amyotrophic lateral sclerosis (ALS), but is dependent on patient effort and bulbar strength. We aimed to use electric impedance tomography (EIT), an emerging non-invasive imaging modality, to measure dynamic lung volume changes.
Methods: Twenty-one patients with ALS underwent sitting and supine spirometry for forced vital capacity (FVC), and sitting and supine EIT.
Evaluation of the perception of information from ChatGPT in myopia education: Perspectives of students and professionals.
Ophthalmic Physiol Opt
January 2025
ISEC LISBOA-Instituto Superior de Educação e Ciências, Lisbon, Portugal.
Purpose: The purpose of this study was to compare the perception and understanding of the information provided by ChatGPT regarding myopia among optometry students, optometrists undertaking a Master degree and practicing optometrists.
Methods: This was a cross-sectional descriptive study using a structured questionnaire distributed via Wooclap to 225 participants (125 optometry students, 21 Masters students and 79 practicing optometrists). All participants evaluated the responses generated by ChatGPT Version 4.
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