The use of high-performance liquid chromatography (HPLC) revealed four previously unreported profiles within a group of mycobacteria consisting of 14 clinical isolates. These mycobacteria, whose identification by conventional tests appeared problematic, mostly resembled Mycobacterium avium complex or Mycobacterium simiae. Genetic analysis revealed, within this group, six different nucleic acid sequences in a hypervariable 16S rRNA segment, but all the isolates appeared to be phylogenetically related to M. simiae. Six isolates representing the largest of groups defined by means of genetic sequencing turned out to belong to the newly described species Mycobacterium lentiflavum. Furthermore, three such clusters precisely coincided with three of those defined by HPLC, while the three remaining clusters shared almost identical HPLC profiles. All but one strain (which, although clearly not belonging to the M. avium complex, hybridized with specific commercial DNA probes) showed high-grade resistance to the majority of antimycobacterial drugs. Three of the isolates were clinically significant according to stringent criteria. Sophisticated techniques, like genetic sequencing or HPLC, by now seem indispensable for differentiating unusual and new mycobacteria from well-established ones.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC229653 | PMC |
| http://dx.doi.org/10.1128/jcm.35.3.697-702.1997 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nontuberculous Mycobacterial Infection and Pulmonary Disease in Northern Israel: A Focus on Mycobacterium simiae.
Chest
December 2024
The B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa; Pulmonology Institute, Carmel Medical Center, Haifa, Israel; Cystic Fibrosis Center, Carmel Medical Center, Haifa, Israel. Electronic address:
Background: The prevalence of nontuberculous mycobacteria (NTM) pulmonary disease is rising globally. Previously, we described a high prevalence of Mycobacterium simiae isolation in Israel.
Research Question: What is the prevalence of NTM pulmonary disease and M simiae pulmonary disease in Northern Israel? Which factors are associated with mortality among people with NTM?
Study Design And Methods: We retrospectively recorded all samples from Clalit Health Services-Haifa District reference mycobacteria laboratory for growth of NTM between January 2010 and March 2021.
Microbial rrn copy number is associated with soil C: N ratio and pH under long-term fertilization.
Sci Total Environ
December 2024
Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; The Rural Development Academy at Zhejiang University, Zhejiang University, Hangzhou 310058, China. Electronic address:
Soil microbial life-history strategies, as indicated by rRNA operon (rrn) copy numbers, strongly influence agro-ecosystem functioning. Long-term N fertilization causes strong and lasting changes in soil properties, yet its impact on microbial strategies remains largely unexplored. Using long-term field experiments across three agro-ecosystems, we consistently found that N fertilization strongly decreased soil C: N ratio and pH, further increasing the community-level rrn copy number, including both average rrn copy number and total 16S rRNA copy number.
View Article and Find Full Text PDFBeyond the Needle: Unmasking Atypical Mycobacteria in Postvaccination Abscesses in Children.
J Indian Assoc Pediatr Surg
July 2024
Amardeep Multispeciality Children Hospital and Research Centre, Ahmedabad, Gujarat, India.
Article Synopsis
- Atypical or nontuberculous mycobacteria (NTM) are environmental organisms linked to opportunistic infections, particularly in immunocompromised patients, with rapid-growing NTM commonly associated with hospital-acquired infections.
- This retrospective study analyzed 13 cases of injection abscess caused by NTM in children over six years, noting that these patients were otherwise healthy, with no chronic illnesses, and all had received standard vaccinations.
- The findings highlight the importance of suspecting NTM infections in patients not responding to treatment, emphasizing the need for strict aseptic techniques to prevent contamination in healthcare settings.
From Triumph to Trial: A Case Study of Non-Tuberculous Mycobacterium After Tetralogy of Fallot (TOF) Correction in an Immunocompetent Child.
Cureus
June 2024
Internal Medicine, Rehman Medical Institute, Peshawar, PAK.
, a slow-growing non-tuberculous mycobacterium (NTM), presents diagnostic challenges due to its resemblance to and other NTMs. While NTM infections and tuberculosis share clinical and radiological features, their management strategies differ. Accurate differentiation is pivotal, as misdiagnosing NTM infections such as MDR-TB can lead to ineffective treatments.
View Article and Find Full Text PDFDifferential radiological features of patients infected or colonised with slow-growing non-tuberculous mycobacteria.
Sci Rep
June 2024
Department of Internal Medicine, Infectious Diseases, Goethe University, University Hospital Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt, Germany.
Article Synopsis
- Non-tuberculous mycobacterial pulmonary disease (NTM-PD) is an increasing health issue, mainly caused by slow-growing mycobacteria (SGM) in Europe.
- A study analyzed 135 CT scans from 84 patients to investigate the correlation between clinical data and radiological features of various SGM species, especially emphasizing Mycobacterium avium complex.
- Findings suggest that patients with M. intracellulare had more severe CT results, and the study indicates that a specific CT scoring system could be beneficial for tracking disease progression and treatment decisions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!