To determine the prevalence of bacteremia caused by Bartonella henselae in domestic cats in the region of Freiburg, Germany, we investigated culture of blood from 100 cats from 89 different households over a 12-month period. B. henselae could be isolated from 13% (13 of 100) of these cats. In eight households with two cats each and in one household with three cats, B. henselae bacteremia was found either in all of the animals or in none of the animals. Positive cultures were more likely to be found for female, young (24 months of age or younger) cats than for male or older cats. Identification of the Bartonella isolates was made by colony morphology, by Gram staining, biochemically by RapID ANA II or Rapid ID 32 A systems, and by whole-cell fatty acid analysis. Differentiation between B. henselae and Bartonella quintana was only possible by 16S rRNA sequencing, enterobacterial repetitive intergenic consensus (ERIC)-PCR and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Genomic fingerprinting of the B. henselae isolates by ERIC-PCR yielded two different patterns based on three distinct bands.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC229631 | PMC |
| http://dx.doi.org/10.1128/jcm.35.3.584-587.1997 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular Detection of Tick-Borne Bacterial Pathogens in Patients With Undifferentiated Febrile Illness in India Using a Flow Chip Assay.
Cureus
December 2024
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, New Delhi, IND.
Background: Tick-borne diseases (TBDs) play a crucial role in human morbidity and mortality, as ticks are highly effective in spreading diseases by transmitting harmful pathogens to humans and animals. The last few decades have seen an increase in the number of recognized tick-borne pathogens and the incidence of TBD worldwide. Several of these diseases are ubiquitous in India.
View Article and Find Full Text PDFDevelopment of Multiplex Assays for the Identification of Zoonotic Species.
Pathogens
December 2024
Intracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
More than one-hundred species that affect animals and humans have been described, eight of which have been associated with emerging and underdiagnosed zoonoses. Most diagnostic studies in humans have used serology or molecular assays based on the 18S rRNA gene. Because the 18S rRNA gene is highly conserved, obtaining an accurate diagnosis at the species level is difficult, particularly when the amplified DNA fragment is small.
View Article and Find Full Text PDFAn integrative taxonomic approach reveals two putatively novel species of phlebotomine sand fly (Diptera: Psychodidae) in Thailand.
Parasit Vectors
January 2025
Center of Excellence in Veterinary Parasitology, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Background: The subfamily Phlebotominae comprises 1028 species of sand fly, of which only 90 are recognized as vectors of pathogenic agents such as Trypanosoma, Leishmania, and Bartonella. In Thailand, leishmaniasis-a sand fly-borne disease-is currently endemic, with 36 documented sand fly species. However, many cryptic species likely remain unidentified.
View Article and Find Full Text PDFTaqMan Array Card real-time polymerase chain reaction panel to detect pathogens in whole blood of febrile inpatients in northern Tanzania, 2016-2019.
Trop Med Int Health
December 2024
Kilimanjaro Christian Medical Centre, Moshi, Tanzania.
Background: Acute febrile illness is a common reason for seeking healthcare in low- and middle-income countries. We describe the diagnostic utility of a TaqMan Array Card (TAC) real-time polymerase chain reaction (PCR) panel for pathogen detection in paediatric and adult inpatients admitted with febrile illness.
Methods: In this prospective cohort study, we screened medical admissions for a tympanic temperature ≥38.
Molecular investigation of Coxiella burnetii and Bartonella in heart valve specimens of patients with endocarditis in Iran.
J Infect Public Health
January 2025
National Reference Laboratory for Plague, Tularemia and Q fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran; Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran. Electronic address:
Article Synopsis
- Infective endocarditis (IE) is a serious health issue, and this study investigates the presence of Coxiella burnetii and Bartonella spp. in heart valves in Iran.
- The research examined 146 patients who had heart valve surgery and found C. burnetii in 12 patients and Bartonella quintana in 15 patients, with two new C. burnetii genotypes identified.
- The study suggests that more research is needed to improve prevention, diagnosis, and treatment methods for Q fever endocarditis and Bartonella endocarditis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!