Visualization of liver sinusoidal endothelial cell repair behavior after preservation by in vitro time-lapse video microscopy.

Sinusoidal endothelial cells are significantly more vulnerable to cold storage and reperfusion than hepatocytes. In this study, a method for assessing the repair behavior of sinusoidal endothelial cells in vitro, after preservation, was investigated. Time-lapse video microscopy analysis was performed and migration rates, division rates, and cell detachment rates were determined. Preservation intervals between 3 and 24 hr and reoxygenation times between 4 and 24 hr were compared. A comparison between sinusoidal endothelial cultures that were stored for 6 hr in University of Wisconsin solution and nonpreserved control cultures was performed. This method allows the investigation of the repair capability of individual cells in vitro. Indications of the kind of preservation/reoxygenation injury that occurs after treatment with several preservation solutions and the resultant repair behavior can be obtained.